青龙衣冷、热乙醇提取物对H22小鼠肿瘤细胞膜生化功能影响的研究  

Study on the effects of cool and hot ethanol extracts from Qinglongyi on the biochemical function of tumor membrane in H22 mice

作　　者：季宇彬[1] 汲晨锋[1] 马宏图[1] 

机构地区：[1]哈尔滨商业大学药物研究所,黑龙江哈尔滨150076

出　　处：《中国中药杂志》2005年第7期531-534,共4页China Journal of Chinese Materia Medica

基　　金：黑龙江省青年基金资助项目(QC02C46)

摘　　要：目的:研究青龙衣的冷、热乙醇提取物对H22小鼠肿瘤细胞膜蛋白含量、膜脂流动性、膜封闭度的影响。方法:应用SDSPAGE电泳分析膜蛋白含量,按Skinitzky法测定膜脂流动性,按Zamudio法测定膜封闭度。结果:青龙衣的冷、热乙醇提取物可降低肿瘤细胞膜蛋白含量,降低肿瘤细胞膜脂流动性,降低肿瘤细胞膜封闭度。结论:青龙衣冷、热乙醇提取物改变了肿瘤细胞膜的生化物质及生化功能,其结果导致肿瘤细胞的解体和死亡,这可能是青龙衣抗肿瘤作用的机制之一。Objective: To study the effects of cool and hot ethanol extracts from Qinglongyi on tumor membrane protein content, lipid fluidity, and membrane close capability in H22 mice. Method: The membrane protein content,by,lipid fluidity and membrane close capability were measured by means of SDS-PAGE, skinitzky assay and Zamudio method respectively. Result: The cool and hot ethanol extracts from Qinglongyi decreased the content of tumor membrane protein, the lipid fluidity and membrane close capability. Conclusion: The cool and hot ethanol extracts from Qinglongyi can change the biochemical substance and biochemical function to cause disaggregation and death of the tumor cell,which may be one of the mechanisms underlying the anti-tumor of Qinglongyi.

关 键 词：肿瘤细胞膜 乙醇提取物 生化功能 青龙衣 影响的研究 H22小鼠 SDS-PAGE 细胞膜脂流动性 蛋白含量 细胞膜封闭度 抗肿瘤作用 电泳分析 生化物质 测定 

分 类 号：R73-36[医药卫生—肿瘤]