WO-1生物衍生骨缓释材料抗感染的实验研究  

EXPERIMENTAL STUDY OF BONE INFECTION ON WO-1 CONTROLLED-RELEASE SYSTEM

作　　者：梁军[1] 杨志明[1] 罗静聪[1] 李秀群[1] 

机构地区：[1]四川大学生物治疗国家重点实验室.干细胞与组织工程研究室

出　　处：《中国修复重建外科杂志》2005年第5期369-372,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划 (863)资助项目(2 0 0 2 AA2 0 50 1 1; 2 0 0 1 AA2 1 60 1 1 );国家自然科学基金资助项目(39830 1 0 0 );卫生部临床学科重点资助项目 (2 0 0 1 3546)~~

摘　　要：目的　研制具有抗感染作用的WO- 1生物衍生骨缓释材料。　方法　应用 型胶原和同种骨制备胶原生物衍生骨复合材料,将WO- 1吸附于胶原生物衍生骨构建成WO- 1生物衍生骨缓释材料,扫描电镜观察其形貌特征;将3H-四环素( 4 5 .1GBq/ mmol)以WO- 1标准品溶液稀释,吸附于胶原生物衍生骨,测定缓释材料中WO- 1的体外释放;将WO- 1生物衍生骨缓释材料置入接种金黄色葡萄球菌、大肠杆菌和绿脓杆菌的培养基中,检测其体外抑菌能力。将WO- 1生物衍生骨植入2 4只新西兰大白兔桡骨缺损处,术后9、16、2 3及30 d各处死2只兔测定血中WO- 1的浓度,以及材料周围肌肉与骨中的WO- 1的浓度。　结果　WO- 1生物衍生骨复合材料保留了天然骨的三维结构,表面有胶状膜覆盖;在体外释放实验中第1天呈暴发释放,以后呈恒定释放;对金黄色葡萄球菌、大肠杆菌等骨感染的常见致病菌有持久稳定的抑菌能力。体内实验中周围骨组织及肌肉组织中WO- 1在各时间点均保持较高的浓度,组间比较差异无统计学意义( P>0 .0 5 ) ,而动物血中WO- 1浓度较低,与骨及肌肉组织中WO- 1浓度相比,差异有统计学意义( P<0 .0 5 )。　结论　WO-Objective To develop a new tissue engineering bone material which has an anti-infective function. Methods Collagen loaded bio-derived bone material was made by using type-I collagen and allograft bone. WO-1 was absorbed to collagen loaded bio-derived bone, then the morphological feature of the new bone material was observed by scanning electronic microscopy. 3H-tetracycline was diluted by WO-1 solution, and was absorbed to collagen loaded bio-derived bone,then the releasing kinetics of WO 1 was detected by 3H tetracycline in vitro. WO-1 bio-derived bone material was grafted into a culture medium with staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa to observe its bacteriostasis ability. WO 1 bio derived bone material was grafted into radius of defected rabbits, the concentration of WO 1 was detected on the 9th, 16th, 23th, and 30th day by HLPC in blood, in bone and in muscle. The bacteriostasis ability of WO 1 loaded bio derived bone was tested in vitro and in vivo. Results WO 1 loaded bio derived bone maintained natural network pore system and the surface of network pore system was coated with collagen membrane. The release of WO 1 from WO 1 loaded bio derived bone showed bursting release on the 1st day, then showed stable release. WO 1 loaded bio derived bone showed lasting and stable bacteriostasis to common pathogens of orthopaedic infections. The high concentration of WO 1 was observed in bone tissue and in muscle tissue at different time points and the difference among groups had no significance( P >0.05), while the concentration of WO 1 in blood was very low( P <0.05). Conclusion WO-1 loaded bio-derived bone has good capability of drug controlled-release and bacteriostasis.

关 键 词：生物衍生骨 WO-1 缓释材料 实验研究 金黄色葡萄球菌 扫描电镜观察 抑菌能力 新西兰大白兔 体外释放实验 复合材料 大肠杆菌 肌肉组织 抗感染作用 常见致病菌 周围骨组织 Ⅰ型胶原 溶液稀释 绿脓杆菌 桡骨缺损 三维结构 

分 类 号：R318.08[医药卫生—生物医学工程] R318.17[医药卫生—基础医学]