直视下袖套法加套入式吻合大鼠全血供肝移植模型建立  

TO RECONSTRUCT THE BLOOD SUPPLY OF HEPATIC ARTERY BY A MODIFIED ARTERIAL “SLEEVE” ANASTOMOSES

作　　者：孟珂伟[1] 吕毅[1] 于良[1] 刘昌[1] 仵正[1] 吴胜利[1] 王博[1] 史源[1] 刘锋[1] 李晖[1] 潘承恩[1] 

机构地区：[1]西安交通大学第一医院肝胆外科

出　　处：《中国修复重建外科杂志》2005年第5期389-391,共3页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的　直视下应用套入式吻合方法重建大鼠移植肝的动脉血供。　方法　SD- SD大鼠肝移植30只,SD- Wistar大鼠肝移植5 0只。供体从腹腔干动脉开始分别结扎脾动脉、胃左动脉、胃右动脉及胃十二指肠动脉,保留肝固有动脉。以8- 0无损伤缝线作袖套法加套入式方法行供体的腹腔干动脉和受体的右肾动脉吻合,恢复移植肝动脉血供。　结果　无肝期最短13min,最长2 1min。套入式吻合肝动脉时间平均4 .0 0±1.31min;手术成功率96 .3% ;SD- SD大鼠成功2 9只,术后不使用免疫抑制剂均健康存活,最长目前已超过2个月。SD- Wistar大鼠成功4 8只,一般术后3～5 d出现急性排斥反应,不用免疫抑制剂于9d后死于排斥反应所致的肝功能衰竭。　结论　直视下袖套法加套入式吻合方法重建大鼠移植肝的动脉血供是一种稳定。Objective To reconstruct the blood supply of hepatic artery of the recipient rat by a modified arterial “sleeve” anastomoses. Methods SD SD and SD Wistar rats liver transplantation were performed in 30 and 50 cases, respectively. The donor splenic artery, left gastric artery, right gastric artery and gastroduodenal artery were ligated, meanwhile the proper hepatic arteries were reserved. The celiac trunk of donors and the stump of right kidney artery of recipient were anastomosed by using 8 0 suture with “sleeve” technique. Results The mean time of artery anastomoses was 4.00±1.31 min.The rate of success was 96.3%. The longest survival time in the model SD SD(29 survived) was more than 2 months. In SD Wistar(48 survived), acute rejectiion was observed 3 5 days after operation and the mean survival time of rat was 9 d. Conclusion The modified arterial “sleeve” anastomoses is an effective method to reconstruct blood supply of hepatic artery of rat recipient in rat liver transplantation.

关 键 词：肝移植模型 袖套法 直视下 Wistar大鼠 胃十二指肠动脉 免疫抑制剂 急性排斥反应 吻合方法 SD大鼠 肝固有动脉 肝动脉血供 套入式吻合 手术成功率 肝功能衰竭 胃左动脉 胃右动脉 动脉吻合 移植肝 腹腔干 脾动脉 无损伤 

分 类 号：R-332[医药卫生]