抑胶素对大鼠脑胶质瘤的抑瘤效应及对神经细胞粘附分子的影响  

The etfects of antigliomatin on NCAM expression and rat brain glioma

作　　者：崔俐[1] 林卫红[1] 姜慧轶[1] 王绍[2] 张淑琴[1] 

机构地区：[1]吉林大学第一医院神经科,吉林长春130021 [2]吉林大学基础医学院生理教研室,吉林长春130021

出　　处：《中风与神经疾病杂志》2005年第2期128-130,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　研究抑胶素对大鼠脑胶质瘤及神经细胞粘附分子的影响。方法　采用脑立体定向术,在Wistar大鼠脑尾状核接种C6细胞,观察大鼠的生存状态,给药7d后观察肿瘤体积变化及抑胶素、顺铂对鼠脑胶质瘤作用的形态学变化,应用免疫组化法检测用抑胶素前后鼠脑胶质瘤神经细胞粘附分子的变化。结果　实验组大鼠有较好的生存状态,抑胶素对C6鼠脑胶质瘤的生长具有明显的抑制作用,经抑胶素治疗后可见肿瘤内神经细胞粘附分子增加。结论　抑胶素是通过抑制胶质细胞瘤生长,提高神经细胞活性,使神经细胞粘附分子增加,从而抑制恶性胶质瘤细胞体外侵袭能力。Objective To study the effect of antigliomatin on the growth of rat brain glioma and the expression of neural cell adhesion molecule (NCAM).Methods C6 glioma cells were injected into the caudate nucleus of rats stereotaxically.The growth of rats was observed after injection.7 days after the treatment of antigliomatin and CDDP,the volume and morphological change of rat glioma were observed and the NCAM expression was detected by immunohistochemical methods.Results Compared with the control group,the growth of rats was much better in the treatment group.The tumor volume of rat glioma was decreased and the expression of NVAM was increased in drug-treatment group.Conlusion It suggests that antigliomatin can inhibit the growth of rat brain glioma by inhibiting the glioma cell viability,increasing the neuronal cell viability,and up-regulating the expression of NCAM.

关 键 词：神经细胞粘附分子 脑胶质瘤 抑瘤效应及 Wistar大鼠 免疫组化法检测 脑立体定向术 生存状态 形态学变化 胶质细胞瘤 胶质瘤细胞 体积变化 C6细胞 脑尾状核 抑制作用 细胞活性 侵袭能力 实验组 肿瘤内 治疗后 生长 

分 类 号：R730.264[医药卫生—肿瘤] R739.41[医药卫生—临床医学]