固生蛋白tenascin对肝细胞癌血管生成及浸润转移的影响  

The effect of tenascin on the angiogenesis , invasion and metastasis of hepatocellular carcinoma

作　　者：李兴睿[1] 廖晓峰[1] 郭悦青[1] 易继林[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030

出　　处：《中华普通外科杂志》2005年第4期213-215,共3页Chinese Journal of General Surgery

摘　　要：目的　探讨固生蛋白(tenascin ,TN)在肝细胞癌(hepatocellularcarcinoma ,HCC)中的表达和与HCC血管生成及浸润转移的关系。方法　应用免疫组织化学法检测4 2例HCC、10例肝硬化和7例正常肝组织内TN的表达情况,观察与HCC病理学特点及微血管密度(microvesseldensity ,MVD)之间的关系。结果　①TN在HCC中的阳性表达率和强度显著高于肝硬化和正常肝脏组织( χ2 =4 15 ,P <0 0 5 ;t=2 4 73,P <0 0 5 ) ;②TN在有包膜侵犯组、病理分级Ⅲ～Ⅳ组及有转移HCC中的阳性表达率及强度明显高于无包膜侵犯组( χ2 =5 4 7,P <0 0 5 ;t=2 138,P <0 0 5 )、病理分级Ⅰ～Ⅱ级组( χ2 =6 87,P <0 0 1;t=2 4 79,P <0 0 5 )以及无转移组( χ2 =10 6 ,P <0 0 1;t=2 6 93,P <0 0 1) ;③肝癌>5cm、有包膜侵犯、有转移、病理分级Ⅲ～Ⅳ级组的MVD值明显高于肝癌≤5cm(t=2 0 36 ,P <0 0 5 )、无包膜侵犯(t =2 32 8,P <0 0 5 )、无转移(t =2 4 94 ,P <0 0 5 )及病理分级Ⅰ～Ⅱ级组(t =2 2 71,P <0 0 5 )。④TN阳性表达HCC中的MVD值明显高于TN阴性表达HCC中的MVD值(t =2 4 87,P <0 0 5 )。Objective To explore the expression of tenascin in hepatocellular carcinoma (HCC). Methods With the method of immunohistochemical staining, specimens from 42 HCC patients, 10 hepatic cirrhotics and 7 normal liver controls were studied. The relationship between the tenascin expression and pathological features as well as microvessel density (MVD) was evaluated. Results The expression of tenascin in the HCC tissues was much higher than that in the non-HCC tissues ( χ 2=4.15, P <0.05; t =2.473, P <0.05). Over expression of tenascin in HCC was related to the invasion of tumor envelop ( χ 2=5.47, P <0.05; t =2.138, P <0.05), Edmondson grades ( χ 2=6.87, P <0.01; t =2.479, P <0.05) and tumor metastasis ( χ 2=10.6, P <0.01; t =2.693, P <0.01). MVD in HCC was associated with tumor size ( t =2.036, P <0.05),the invasion of tumor envelop ( t =2.328, P <0.05), tumor metastasis ( t =2.494, P <0.05) and Edmondson grades( t =2.271, P <0.05). MVD of HCC with positive expression of tenascin was much higher than that in HCC with negative expression of tenascin ( t =2.487, P <0.05). Conclusion Tenascin may play a crucial role in the angiogenesis, invasion and metastasis in cases of HCC.

关 键 词：浸润转移 血管生成 TENASCIN 肝细胞癌 蛋白 carcinoma 免疫组织化学法 阳性表达率 病理分级 HCC 0.05 微血管密度 病理学特点 方法应用 表达情况 肝脏组织 阴性表达 表达上调 肝硬化 TN 侵犯 包膜 组织内 转移组 D值 

分 类 号：R735.7[医药卫生—肿瘤]