反义及正义Vim重组逆转录病毒表达载体的构建  

Construction of Antisense and Sense Vimentin Retrovirus Expression Vector

作　　者：林江凯[1] 蔡文琴 

机构地区：[1]第三军医大学附属西南医院神经外科,重庆400038 [2]第三军大学基础部组胚教研室

出　　处：《华南国防医学杂志》2005年第1期21-24,F003,共5页Military Medical Journal of South China

基　　金：国家自然科学基金资助项目(30471781)

摘　　要：目的构建反义及正义波形蛋白重组逆转录病毒载体,研究波形蛋白在反应性胶质化中的功能与作用。方法应用分子克隆方法克隆、鉴定、包装并感染培养的星形胶质细胞。结果同时获得全长反义及正义Vim逆转录病毒克隆细胞株和较高滴度的病毒上清,反义Vim逆转录病毒抑制培养星形细胞的Vim表达。结论重组的反义及正义Vim逆转录病毒可用于进一步的实验研究。Objective To construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis. Methods Molecular clone methods were used for tp clone,identifing the antisense and sense vimentin retrovirus and their package.The astrocytes were infected by retrovirus,cultured and determined by SABC method. Results Both the antisense and sense vimentin retrovirus were gained and also high titer of retrovirus superfactant was gotten. The expression of vimentin in the cultured astrocytes was inhibited by antisense vimentin retrovirus compared with sense retrovirus. Conclusion Antisense and sense vimentin retrovirus can be used for the study of reactive gliosis.

关 键 词：重组逆转录病毒表达载体 VIM 正义 重组逆转录病毒载体 反应性胶质化 星形胶质细胞 波形蛋白 克隆细胞株 分子克隆 方法应用 星形细胞 病毒抑制 实验研究 高滴度 培养 

分 类 号：R394[医药卫生—医学遗传学] R651.15[医药卫生—基础医学]