氪激光诱发大鼠脉络膜新生血管的基因表达谱  

Gene expression patterns of choroidal neovascularization induced by krypton laser photocoagulation

作　　者：陈学国[1] 何守志[1] 

机构地区：[1]解放军总医院眼科,100853

出　　处：《中华眼科杂志》2005年第4期317-320,共4页Chinese Journal of Ophthalmology

基　　金：全军十五课题基金资助项目(02M014)

摘　　要：目的　研究氪激光诱发大鼠脉络膜新生血管的基因表达谱。方法　对氪激光诱发BN大鼠脉络膜新生血管的脉络膜组织和正常BN大鼠的脉络膜组织进行总RNA的提取,将纯化后的mRNA进行逆转录制备杂交探针,应用含有5705条OligoDNA的表达谱芯片对氪激光诱发BN大鼠脉络膜新生血管的脉络膜组织和正常BN大鼠的脉络膜组织进行差异表达谱分析。结果　在氪激光诱发BN大鼠脉络膜新生血管的基因表达谱中差异表达基因共有46条,其中上调的基因3条,下调的基因43条。它们分别是物质转运、信号传导、代谢、发育、分化、细胞黏附相关基因。结论　氪激光诱发脉络膜新生血管的发生发展涉及多基因改变。Objective To explore the gene expression patterns of choroidal neovascularization with gene chip technology. Methods Total RNA was isolated from normal choroid and choroid tissues with neovascularization induced by krypton laser photocoagulation in Brown Norway rat. The mRNA was purified and was reversely transcribed to cDNA to prepare for hybridization probes. In choroidal neovascularization, the differentially expressed genes were assayed with gene chips which contained 5705 rat genes. Results There were 46 genes expressed differentially in choroidal neovascularization. Of these, the expression of 3 genes was upregulated and 43 genes downregulated. These genes were involved in the function of transport, signal, metabolism, development and differentiation of cell adhesion. Conclusion Numerous genes are involved in the development of choroidal neovascularization induced by krypton laser photocoagulation.

关 键 词：氪激光 诱发作用 大鼠 脉络膜 血管 基因表达谱 寡核苷酸序列 

分 类 号：R773.4[医药卫生—眼科]