胃癌细胞周期G1/S转换期基因表达谱的分析  

Analysis of gene expression profile of G1/S transition in gastric cancer cell cycle

作　　者：兰斌[1] 刘炳亚[1] 张济 王侃侃 陈雪华[1] 朱正纲[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科上海消化外科研究所 [2]上海市血液病研究所

出　　处：《中华胃肠外科杂志》2005年第3期229-233,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家973重点基础研究发展规划基金(2002CB713700)

摘　　要：目的检测胃癌细胞在DNA合成前期/DNA合成期(G1/S)交界点基因表达谱的变化,探讨胃癌细胞由G1期进入S期相关基因的调控作用。方法应用胸腺嘧啶核苷(thymidine)-偌考达唑(nocodazole)及双胸腺嘧啶核苷(doublethymidine)法分别阻断胃癌细胞株(MKN45)于DNA合成后期/有丝分裂期(G2/M)及G1/S交接点释放后收集标本,应用cDNA微阵列基因芯片检测细胞周期中G1早期、G1末期、S早期及S晚期的基因表达谱,专业软件进行聚类分析。结果检测到4个时间点均可信的2001个基因,其中959个基因出现上调或下调。147个基因在G1末期出现上调,其功能涉及DNA代谢、转录与翻译、翻译后修饰、泛素化、信号转导等,这些过程在不同环节上影响细胞周期。结论多基因参与胃癌细胞周期G1期至S期的转换,且为其转换所必需,部分参与以上过程的基因与胃癌过度增殖有关。Objective To detect the variation of gene expression profile of G1 /S transition and elucidate the role of related genes regulating cell cycle from G1 to S phase in gastric cancer. Methods Nocodazole thymidine and double thymi dine methods were used to synchronize gastric cells at G2/M and G1/S point,cDNA microarray chips was applied to examine the gene expression profile at G1 early and late phase,S early and late phase during the cell cycle,hierarchy analysis was conducted by a professional software package. Results A total of 2001 genes were detected availably,959 genes appeared to be upregulated or downregulated,i ncluding 147 genes upregulated at G1 late phose. These 147 genes are involved in DNA metabolism,transcription and translation,posttranslational modification,ubi quitination,signal transduction etc,which all affected cell cycle from different aspects. Conclusions Complex multiplegene processes,such as DNA metabolism,tran scription and translation,posttranslational modification,ubiquitination,signal t ransduction etc,are implicated in and also essential for G1/S transition during gastric cancer cell cycle,part of these genes are significantly associate with o verproliferation in gastric cancer.

关 键 词：基因表达谱 癌细胞周期 G1/S转换 胸腺嘧啶核苷 DNA合成 cDNA微阵列 基因芯片检测 有丝分裂期 胃癌细胞株 DNA代谢 调控作用 相关基因 聚类分析 专业软件 信号转导 过度增殖 G1期 泛素化 多基因 S期 早期 末期 上调 出现 

分 类 号：R735.2[医药卫生—肿瘤]