铬营养状况对Wistar大鼠体重及血清激素水平的影响  

Effect of chromium nutriture on body weight and serum hormone in Wistar rats

作　　者：徐贵发[1] 刘清君 赵秀兰[1] 朱凤[1] 李辉[1] 

机构地区：[1]山东大学公共卫生学院营养与食品卫生研究所,山东济南250012 [2]中国疾病控制预防中心,北京100050

出　　处：《山东大学学报（医学版）》2005年第4期291-293,共3页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30170803)

摘　　要：目的:探讨铬的营养状况对生长发育期Wistar大鼠体重及其血清瘦素、胰岛素、生长激素和肿瘤坏死因子水平的影响。方法:将40只体重(100±10)g断乳1周的雄性Wistar大鼠随机分为缺铬组、低剂量补铬组(20μg/kg体重)、中剂量补铬组(200μg/kg体重)和高剂量补铬组(2000μg/kg体重)。各组大鼠均喂饲缺铬饲料,饮用去离子水。补铬组大鼠每日经灌胃给予三氯化铬。每周称重1次,并于45d后断头取血,用石墨炉原子吸收法测量大鼠血清中铬的含量;同时用放免法检测血清中瘦素、胰岛素、生长激素及肿瘤坏死因子水平。结果:大鼠体重与血清铬水平呈负相关(P<0.05)。缺铬组大鼠血清中瘦素、胰岛素高于高剂量组(P<0.05)、肿瘤坏死因子水平高于中剂量组和高剂量组(P<0.01),生长激素水平低于中剂量组和高剂量组(P<0.05)。结论:铬缺乏可以改变大鼠体重和血清激素水平。Objective: To study the effect of chromium nutriture on body weight, level of serum leptin, insulin, growth hormone and tumor necrosis factor in developmental Wistar rat. Methods: Forty Wistar rats were randomly divided into four groups: one was chromium deficiency group, the other three were supplied with CrCl3 (20 μg/kg, 200 μg/kg and 2 000 μg/kg, respectively) by gavage. All rats in four groups were fed on chromium deficiency diet and weighted once a week. Rats were killed and collected blood after 45 days .The chromium contents of the serum was measured by furnace atomic absorption spectrometry. The concentrations of serum leptin, insulin, growth hormone and tumor necrosis factor were tested by RIA method. Results: The chromium contents in serum of rats had negative correlation with body weight. The leptin and insulin levels of the chromium deficiency group were significantly higher than the those of high-dose group, and the tumor necrosis factor level was significantly higher than that of the middle-dose group and the high-dose group, while the growth hormone level of the chromium deficiency group was significantly lower than that of the middle-dose group and the high-dose group. Conclusion: Chromium deficiency may change the body weight and the level of serum hormone of Wistar rats.

关 键 词：铬 瘦素 胰岛素 生长激素 肿瘤坏死因子 大鼠 Wistar 

分 类 号：R151.1[医药卫生—营养与食品卫生学] R-332[医药卫生—公共卫生与预防医学]