人参皂甙对肺癌细胞和血管内皮细胞增生、凋亡及其周期的影响  

Effect of ginsenoside on the cellular proliferation, apoptosis and cell cycles in LC A549 and HUVEC 304 cell lines

作　　者：陈明伟[1] 马爱群[1] 倪磊[2] 黄晨[2] 张殿增[2] 牛小英[2] 

机构地区：[1]西安交通大学医学院第一附属医院第一附属医院,西安710061 [2]西安交通大学医学院环境与疾病相关基因实验室,西安710061

出　　处：《中南大学学报（医学版）》2005年第2期149-152,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金资助项目(30171190)

摘　　要：目的:探讨中药20(R) 人参皂甙Rg3(Rg3)对肺癌细胞和血管内皮细胞增生、凋亡以及细胞周期的影响。方法:进行人肺腺癌细胞株A549、人脐静脉血管内皮HUVEC304细胞培养,不同浓度Rg3干预,采用MTT法、流式细胞术、透射电镜等技术,了解不同浓度Rg3对上述细胞增生、凋亡以及细胞周期的影响。结果: 3×10 6mol/L的Rg3可导致A549细胞明显凋亡,凋亡率为29. 8%,与对照组( 15. 0% )相比差异有统计学意义(P<0. 05)。Rg3对A549细胞周期的影响无显著差异。1×10 4 mol/L的Rg3对内皮细胞的生长抑制率为12. 53%,显著高于其它组(P<0. 05),不同浓度Rg3对条件培养液(CM)诱导的血管内皮细胞的增生均有明显的抑制作用(P<0. 05),电镜下可见10 6 mol/L浓度的Rg3可使CM诱导的内皮细胞出现凋亡小体。结论:适当浓度的Rg3有抗肿瘤作用和抑制肿瘤新生血管生成的作用。Objective To determine the effect of ginsenoside on the cellular proliferation, apoptosis and cell cycles in LC A549 and HUVEC 304 cell lines. Methods A549 and HUVEC 304 cell lines were cultured with different concentrations of ginsenoside. Cellular proliferation was detected with MTT, apoptosis and cell cycles were checked with Flow Cytometer, and change of microstructure was observed by transmission electron microscope. Results The apoptosis rate was 29.8% in A549 cell lines after being interfered with ginsenoside at 3×10 -6 mol/L, significantly higher than that in the control group ( P <0.05). No change was observed in the cell cycles after being interfered with ginsenoside. The inhibitive rate of ginsenoside was 12.53% for HUVEC 304 cell line at 1×10 -4 mol/L ( P <0.05). The cells induced by conditioned medium could be inhibited by ginsenoside, and apoptotic body could be found in cells induced by conditioned medium at 10 -6 mol/L. Conclusion The proliferation of vascular endothelial cell could be inhibited by ginsenoside, and apoptosis could also be found in both tumor cells and cells induced by conditioned medium after being interfered with ginsenoside.

关 键 词：血管内皮细胞增生 肺癌细胞 人参皂甙 肿瘤新生血管生成 细胞株A549 mol/L 细胞周期 不同浓度 A549细胞 流式细胞术 生长抑制率 条件培养液 moL/L 抗肿瘤作用 Rg3 人肺腺癌 细胞培养 人脐静脉 MTT法 透射电镜 抑制作用 

分 类 号：R734.2[医药卫生—肿瘤]