GSTT1基因多态性与中国四川汉族人群肺癌遗传易感性关系的研究  

Relationship between genetic polymorphism of GSTT1 gene and inherent susceptibility to lung cancer in Han population in Sichuan, China

作　　者：袁天柱[1] 周清华[1] 朱文[1] 郭占林[1] 李代蓉[1] 王艳萍[1] 陈晓禾[1] 刘伦旭[1] 杨俊杰[1] 

机构地区：[1]四川大学华西医院四川省肺癌分子重点实验室,胸心外科,成都610041

出　　处：《中国肺癌杂志》2005年第2期107-111,共5页Chinese Journal of Lung Cancer

基　　金：国家"十五"攻关项目基金(No.2001BA703B11)资助~~

摘　　要：背景与目的 GSTs可能参与机体致癌物的解毒反应,如保护个体免受吸烟的损害,因此GSTs基因多态性被认为是个体是否患癌的易感因素.本研究的目的是探讨GSTT1基因多态性与中国四川汉族人群肺癌遗传易感性的关系.方法采用病例对照和PCR-RFLP方法检测中国四川汉族人群肺癌患者150例和健康对照者152例的GSTT1基因缺失型的频率,并评价其与吸烟和肺癌遗传易感性的关系.结果①GSTT1(-)基因型在肺癌组和对照组分别为54.7%(82/150)和38.2%(58/152),二者间比较有显著性差异(OR=1.681,95%CI＝1.009～2.803,P＝0.046);②GSTT1(-)基因型患肺鳞癌 (OR＝2.969, 95%CI＝1.511～5.834,P＝0.002)及肺腺癌(OR＝2.095,95%CI＝1.060～4.140,P＝0.033)的风险性明显增加;③吸烟者中GSTT1(-)基因型者患肺癌的风险是GSTT1(+)者的4.051倍;④GSTT1(-)基因型者中,吸烟者患肺癌的风险是不吸烟者的53.885倍;⑤吸烟≥20包年者中,GSTT1(-)基因型者患肺癌的风险是GSTT1(+)者的4.296倍.结论①GSTT1(-)基因型增加四川汉族人群患肺癌的风险性,特别是增加患肺鳞癌的风险;②GSTT1(-)基因型和吸烟之间存在交互作用,吸烟量越大且为GSTT1(-)基因型者则患肺癌的风险性越大.Background and objective Some studies have supposed that glutathione S-transferases (GSTs) may be involved in detoxification of carcinogens, especially from tobacco smoke. Therefore, polymorphism of GSTs has been considered as potential protectors of individual cancer risk. The objective of this study is to investigate the relationship between genetic polymorphism of GSTT1 and inherent susceptibility to lung cancer in Han population in Sichuan, China. Methods A case-control study was carried out to compare the distribution frequency of GSTT1 gene polymorphism between lung cancer (n=150) and control healthy individuals (n=152) with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and to analyze the relationship between the GSTT1 gene polymorphism and smoking and the inherent susceptibility of lung cancer. Results ① The distribution frequency of GSTT1(-) genotype was 54.7% (82/150) in lung cancer and 38.2% (58/152) in control group respectively (OR=1.681, 95%CI=1.009-- 2.803 , P=0.046); ② GSTT1(-) genotype remarkably increased the risk of squamous cell carcinoma (OR=2.969, 95%CI= 1.511 --5.834, P=0.002) and adenocarcinoma (OR=2.095, 95%CI=1.060--4.140, P= 0.033 ); ③ In smokers, GSTT1(-) genotype significantly increased the risk for lung cancer (OR=4.051, 95%CI=1.959-- 8.380 , P=0.000); ④ In people with GSTT1(-) genotype, smoking markedly increased the risk for lung cancer (OR=53.885, 95%CI=11.789--246.302, P=0.000); ⑤ In heavy smokers (≥20 packyears), GSTT1(-) genotype could remarkably increase the risk of lung cancer (OR=4.296, 95%CI=1.649--11.190, P=0.003). Conclusion ① People with GSTT1(-) genotype have significantly increased risk for lung cancer in Han population in Sichuan, China, especially for squamous cell carcinoma. ② GSTT1(-) genotype interacts synergistically with smoking on lung cancer risk. The more the cigarettes smoke, the higher the risk of lung cancer increases in those people who are smokers with GSTT1(-) genotype.

关 键 词：肺肿瘤 GSTT1 基因多态 肺癌易感性 易感基因 

分 类 号：R734.2[医药卫生—肿瘤]