苯巴比妥对癫大鼠海马苔藓纤维突触重组和认知发育的影响  

Effects of Phenobarbital on Cognition and Synaptic Reorganization of Hippocampal Mossy Fibers in Developmental Epileptic Rats

作　　者：陈燕惠[1] 陈文雄[1] 陈珊[1] 郑耀辉[1] 王丽[2] 

机构地区：[1]福建医科大学附属协和医院儿科,福州350001 [2]北京大学医学部第一临床医院儿科,北京100034

出　　处：《福建医科大学学报》2005年第2期129-132,共4页Journal of Fujian Medical University

摘　　要：目的　探讨苯巴比妥(PB)对不同发育期癫大鼠海马内苔藓纤维(MF)发芽与认知功能的影响。　方法　Wistar大鼠按出生后日龄分组(P5、P15、P2 5、P6 0 ) ,以红藻氨酸(KA)制备癫模型,实验结束取脑制片,行Timm染色,观察海马齿状回内分子层染色改变。认知功能测验采用跳台法。　结果　KA致对P5、P15组海马内MF发芽现象、大鼠学习记忆功能无明显影响,但可使P2 5、P6 0组大鼠海马内MF发芽现象加剧,伴学习、记忆成绩明显下降,PB可加剧这一现象。PB对未致大鼠海马内MF发芽现象和学习记忆功能无明显影响。　结论　海马MF突触重组是PB影响不同发育时期癫大鼠学习记忆功能的重要原因。Objective To investigate the effects of phenobarbital(PB) on cognition and synaptic reorganization of hippocampal mossy fibers(MF) in developmental epileptic rats. Methods Wistar rats according to postnatal day P5, P15, P25, P60 divided into four groups(each group 40 rats). Epileptic models were established by injection of kainic acid(KA). Timm's silver sulfide staining were used to examine the MF sprouting in hippocampal inner molecular layer. Using step down test tested the cognitive function. Results KA administration show no significant different effects on P5 and P15, but elicit the increase of the MF sprouting and cognitive disturbances in older rat(P25 and P60) and PB injection exacerbated the disturbances. Conclusion The synaptic reorganization of hippocampal MF is an important factor in the cognitive disturbances. PB exacerbated the disturbances.

关 键 词：苯巴比妥 癫痫 认知 苔藓纤维 海马 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R338.64[医药卫生—临床医学]