部分去背根促进神经营养因子在初级感觉神经元顺行转运的研究  

Partial Dorsal Root Rhizotomy Increases the Anterograde Transportation of Neunotrophic Factors in Primary Sensory Neuron

作　　者：龙双涟[1] 李咏梅[2] 袁源[2] 王廷华[2] 吴林艳[2] 

机构地区：[1]南华大学医学院组织胚胎学教研室,衡阳421200 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第3期325-327,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 0 0 0 2 2 7)资助

摘　　要：目的　探讨部分去背根是否促进BDNF、NT- 3和GDNF在初级感觉神经元顺行转运。方法　将5只成年猫行单侧部分背根切断术,切除一侧的L1 ～L5,L7～S2 背根神经节(DRG) ,保留同侧L6 DRG为备用根,在L6 背根距DRG 1cm处捆绑双侧背根。术后3d取两侧L6 背根制作2 0μm厚冰冻切片,分别用BDNF、NT- 3、GDNF抗血清按免疫组织化学ABC法染色。观察实验侧和对照侧捆绑背根部位的近DRG端和近脊髓端各因子的免疫反应强度。结果　对照侧捆绑处两端NT- 3、GDNF的免疫反应强度均为弱阳性反应,BDNF捆绑处近DRG端的免疫反应(++)较近脊髓端者强(+)。实验侧捆绑处近DRG端BDNF、NT- 3和GDNF的免疫反应(+++)较捆绑处近脊髓端强,且较对照组近DRG强。结论　部分去背根动物背根近DRG端BDNF、NT- 3和GDNF的免疫反应强度增加可能提示部分去背根可促进BDNF、NT- 3、GDNF从备用背根节神经元顺行转运进入脊髓。Objective To investigate whether partial dorsal root rhizotomy promotes the anterograde transportation of BDNF, NT-3 and GDNF in the primary sensory neuron. Methods Five adult cats were subjected to unilateral spared root rhizotomy (the DRGs of L_1-L_5 and L_7-S_2 were removed, but L_6 DRG was spared) and bilateral dorsal roots of L_6 were ligated at the same time. Three days after operation, dorsal roots were taken out and made into frozen sections 20 μm in thickness. The sections were stained using specific BDNF, NT-3, GDNF antibody (1∶1500) by ABC method. The immunoreactive density was observed in a site near DRG and a site near spinal cord. Results In the control group (with spared L_6 DRG), there were no marked differences in NT-3 and GDNF immunoreactivity between the site near DRG and the site near spinal cord, while BDNF immunoreactivity was more intense in the site near DRG than that in the site near spinal cord. In the operation group, the immunoreactivity of each neurotrophin in the site near DRG was stronger than that in the site near spinal cord, and the immunoreactivities of BDNF, NT-3, GDNF in the site near DRG of the operation were stronger than those of the control group respectively. Conclusion The increasing of immunoreactivities of neurotrophins near DRG following partial dorsal root rhizotomy suggests that partial dorsal root rhizotomy can promote their anterograde transportation from spared DRG to the spinal cord.

关 键 词：部分去背根 神经营养因子 初级感觉神经元 顺行转运 

分 类 号：R33[医药卫生—人体生理学]