STR技术在种用东北细毛羊遗传管理中的应用  

Application of STR on Genetic Management of Northeast Fine-wool Sheep as Breeder

作　　者：金煜[1] 张研[1] 牛黎明[1] 白素英[1] 邵伟庚[1] 徐艳春[4] 李波[4] 马玉堃[4] 

机构地区：[1]东北林业大学野生动物资源学院 [2]1.东北林业大学野生动物资源学院 [3]2.国家林业局野生动植物检测中心,哈尔滨150040 [4]国家林业局野生动植物检测中心

出　　处：《畜牧兽医学报》2005年第5期426-430,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：以10个STR基因座作为遗传标记系统,分析东北细毛羊种群的遗传多样性和亲缘关系。在遗传多样性分析中,群体的平均等位基因数为8.2,平均杂合度为0.794,平均多态信息含量为0.768,遗传多样水平较丰富。在亲缘关系鉴定和确认中,双亲鉴定时,10 个微卫星基因座的累积非父排除概率为0.999 9,平均父权相对机会(RCP)为99.999%;单亲鉴定时,累积非父排除概率为0.996 2,平均RCP为99.993%。根据所选的STR基因座,可建立一套适用于种用东北细毛羊遗传资源分析和亲缘关系鉴定的方法体系,为我国东北细毛羊品种资源的保护和经济性状的筛选提供科学指导。同时,也可为解决东北细毛羊归属纠纷提供一个科学的鉴定方法。The ten STR loci as a system of genetic markers were used to analyze genetic diversity and parentage relationship in Northeast fine-wool sheep group. On average, the number of alleles per locus is 8.2, heterozygosity is 0.794, polymorphism information content (PIC) is 0.768. Northeast fine-wool sheep has abundant genetic diversity. To exclusion probability and Paternity identify, in mother-son-father set, ten markers will exclude the alleged parent with a probability of >0.999 9, and RCP is 99.999%; In the sets without mother sample, these markers will exclude the alleged parent with a probability of >0.996 2, and RCP is 99.993%. On the basis of STR loci selected in this study, it can set up a method for estimating genetic diversity and identifying parentage relationships of Northeast fine-wool sheep, and give a scientific direction to protect the resource of Northeast fine-wool sheep and select economical traits. In addition, it can also provide a method for troubleshooting Northeast fine-wool adscription.

关 键 词：东北细毛羊 种用 STR基因座 亲缘关系鉴定 遗传多样性分析 平均等位基因数 应用 管理 技术 多态信息含量 微卫星基因座 平均杂合度 标记系统 方法体系 资源分析 经济性状 品种资源 鉴定方法 定时 概率 累积 科学 

分 类 号：S826.32[农业科学—畜牧学] S829.1[农业科学—畜牧兽医]