缺氧对高原藏族、汉族人脐静脉内皮细胞VEGF、iNOS和eNOS mRNA表达影响的比较  

Effects of hypoxia on expression of VEGF, iNOS and eNOS mRNA in human umbilical vein endothelial cells from Tibetan and Han

作　　者：高文祥[1] 高钰琪[1] 李素芝[2] 张国斌[1] 宋玲[1] 孙秉庸[1] 史景泉[3] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学研究室,全军高原生理与高原病研究重点实验室,重庆400038 [2]西藏军区总医院,西藏拉萨850001 [3]第三军医大学附属西南医院病理科,重庆400038

出　　处：《中国病理生理杂志》2005年第5期844-848,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30393131)

摘　　要：目的比较缺氧对世居高原藏族人和移居高原汉族人脐静脉内皮细胞(UVECs)血管内皮生长因子(VEGF)、诱导型一氧化氮合酶(iNOS)和内皮型一氧化氮合酶(eNOS)mRNA表达的影响。方法分离脐静脉内皮细胞,体外原代培养,分为①世居高原藏族组和②移居高原汉族组两组,每组包括常氧对照和缺氧(0.5%O2)2h、4h、12h和24h时点。分离细胞总RNA,分别用RT-PCR检测VEGF、iNOS和eNOSmRNA表达。结果缺氧可以诱导世居高原藏族人UVECs表达iNOS和VEGFmRNA表达增加,同时抑制eNOSmRNA表达。移居高原汉族人UVECs表达上述基因mRNA的特点与世居高原藏族人相似。结论缺氧时世居高原藏族人UVECsVEGF、iNOS和eNOSmRNA表达特点与移居高原汉族人相似,提示上述基因表达的改变可能是缺氧反应机制的共同过程。AIM: To compare the effects of hypoxia on expression of vascular endothelial growth factor (VEGF), iNOS and eNOS mRNA in cultured umbilical vein endothelial cells (UVECs) obtained from Tibetan and Han. METHODS: UVECs were obtained from native Tibetan and immigrant Han, respectively and cultured under hypoxia conditions (0.5% oxygen) for 2 h, 4 h, 12 h, and 24 h and normoxic conditions. VEGF, iNOS and eNOS mRNAs were detected with methods of RT-PCT. RESULTS: VEGF and iNOS mRNAs were up-regulated while eNOS mRNA depressed by hypoxia similarly in Tibetan and Han UVECs. CONCLUSION: Our results suggest that the changes of VEGF, iNOS and eNOS mRNA expression are common pathways in the mechanisms of hypoxic responses.

关 键 词：高海拔 西藏 缺氧 内皮生长因子 一氧化氮合酶 

分 类 号：R394[医药卫生—医学遗传学]