组胺H_2受体阻滞剂阻断再生障碍性贫血患者CD_8^+细胞抑制正常CFU-Mk生长的作用  

In vitro inhibitory effect of CD_8^+ cells from patients with aplastic anemia on normal CFU-Mk growth is blocked by cimetidine

作　　者：李明 汪明春 张琼丽 杜新[2] 

机构地区：[1]深圳市血液病研究所,广东深圳518035 [2]深圳市第二人民医院血液科,广东深圳518035

出　　处：《中国病理生理杂志》2005年第5期936-938,共3页Chinese Journal of Pathophysiology

基　　金：广东省卫生厅科学研究基金资助项目(No.A1998551);深圳市科委科学研究基金资助项目(No.199805020)

摘　　要：目的探讨再生障碍性贫血(再障)患者CD8+细胞及其组胺受体对巨核系造血的影响。方法在20例正常人的巨核细胞集落(CFU-Ck)的无血清培养体系中分别加入20例再障患者外周血CD8+细胞、再障患者外周血CD8+细胞+甲氰咪胍和单加甲氰咪胍。结果再障患者外周血CD8+细胞在体外能抑制正常人骨髓细胞CFU-Mk的生长,1.0×10-5mol/L的甲氰咪胍能解除这种抑制作用。1.0×10-5mol/L的甲氰咪胍对正常人骨髓细胞CFU-Mk的生长无抑制作用。结论再障患者CD8+细胞对正常CFU-Mk生长有明显的抑制作用,而组胺H2受体阻滞剂甲氰咪胍能解除这种抑制作用。AIM: To investigate the effect of CD_8+ cells from aplastic anemia (AA) patients and its histamine type II (H_2) receptors on the growth of normal CFU-Mk. METHODS: The effects of CD_8+ cells and/or cimetidine, on normal human CFU-Mk growth were studied by using CFU-Mk assay. RESULTS: The CD_8+ cells from the perpheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-Mk. This inhibitory effect was blocked by cimetidine at concentration of 1.0×10^-5 mol/L. 1.0×10^-5 mol/L cimitidine alone didn't inhibit the growth of normal CFU-Mk. CONCLUSION: H_2 receptor antagonist cimitidine abolishes the suppressive effect of AA patients CD_8+ cells on the growth of normal CFU-Mk.

关 键 词：贫血 再生障碍性 CD8^+细胞 造血干细胞 受体 组胺H2 西咪替丁 

分 类 号：R363[医药卫生—病理学]