雌激素对视网膜血管渗漏的影响  

Effect of estrogen on the permeability of retinal blood vessel

作　　者：张红兵[1] 张军军[1] 马麟[1] 张美霞[1] 赵英杰[1] 

机构地区：[1]四川大学华西医院眼科,成都西安市眼科医院610041

出　　处：《中华眼底病杂志》2005年第3期174-176,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：卫生部基金资助项目 (2 0 0 1 32 1 )

摘　　要：目的　探讨用去势方法研究雌激素影响眼部疾病的可行性以及雌激素对视网膜血管渗漏的影响。　方法　健康成熟的雌性SD大鼠2 2只,随机分为实验组和对照组。实验组雌性SD大鼠用去势方法降低体内雌激素水平;对照组用假去势方法,不影响雌激素水平。手术效果用阴道上皮涂片方法证实。用光动力诱导方法建立视网膜静脉阻塞模型,用分光光度计测量视网膜伊文思蓝渗漏量。　结果　实验组大鼠阴道上皮成熟值降低明显(t =2 1.0 0 8,P =0 .0 0 0 ) ,对照组大鼠成熟值变化不明显(t =0 .3 19,P =0 75 6)。实验组大鼠视网膜平均伊文思蓝渗漏量为(2 5 .5 0 3 0±4.3 7847)ng/mg ,对照组大鼠视网膜平均伊文思蓝渗漏量为(17 .83 0 0±4 2. 65 69)ng/mg。2组之间差异有统计学意义(t =3 . 9693 6,P =0 . 0 0 1)。　结论　去势方法是研究雌激素在眼部疾病作用的可靠模型,雌激素水平降低时视网膜血管的渗漏增加。Objective To explore the effect of estrogen on the permeability of retinal blood vessel by ovariectomy. Methods Twenty-two healthy rats were divided into experimental and control group randomly. Estrogen level of rats decreased due to ovariectomy in the experimental group while stabilized by sham-ovariectomy in the control group. The results were confirmed by vaginal epithelium smearing. Retinal vein occlusion was established by photodynamic method, and leakage of Evan's blue in retina was determined by spectrophotometer. Results Mature value of vaginal epithelium decreased significantly in ovariectomy rats( t=21.008,P =0.000) while not significantly in sham-ovariectomy ones ( t=0.319,P =0.756); the mean leakage of Evans blue was (25.503 0±4.378 47) ng/mg in experimental group, and (17.830 0±4.265 69) ng/mg in the control group, and the difference between the two groups is significant( t=3.969 36,P =0.001). Conclusion Ovariectomy is an useful method to study the effect of estrogen on ocular diseases, and when estrogen level decreases, the permeability of retinal blood vessel increases.

关 键 词：血管渗漏 雌性SD大鼠 雌激素水平 去势方法 静脉阻塞模型 伊文思蓝 眼部疾病 阴道上皮 分光光度计 视网膜血管 对照组 实验组 涂片方法 手术效果 方法建立 成熟 光动力 统计学 mg 平均 

分 类 号：R774.1[医药卫生—眼科]