酸性环境下唾液分泌型IgA对变形链球菌免疫反应的体外研究  

The study of salivary-SIgA reaction to Streptococcus mutans in acid environment

作　　者：聂敏[1] 范华俐[2] 樊明文[2] 胡萍[2] 刘加荣[2] 边专[2] 

机构地区：[1]武汉大学口腔医学院牙体牙髓科,430079 [2]武汉大学口腔医学院科口腔生物医学工程教育部重点实验室,430079

出　　处：《中华口腔医学杂志》2005年第3期215-218,共4页Chinese Journal of Stomatology

摘　　要：目的探讨在酸性条件下,唾液分泌型免疫球蛋白A(SIgA)与变形链球菌的特异性结合是否会产生变化。方法收集武汉市20位志愿者的牙菌斑样本及无菌颌下腺、舌下腺唾液,随机引物聚合酶链反应(AP-PCR)鉴定临床变形链球菌分离株。分组培养:对照组在pH7.2的培养基中厌氧培养,实验组在pH5.5的培养基中培养。变形链球菌菌株全细胞蛋白凝胶电泳,利用Westernblot方法分析个体唾液SIgA与自身变形链球菌菌株和标准参考菌株S.mutansGS-5,S.mutansIngbrittC的免疫印迹反应。结果①SIgA与自身菌株和标准菌株均有免疫印迹条带;②同一个体对不同基因型的变形链球菌菌株出现不同的免疫印迹条带;③酸休克后,没有新的特异性条带出现,但有某些印迹条带强度增强。结论变形链球菌在酸性条件下可以产生酸休克蛋白,但不足以与SIgA发生特异性结合,仅有部分反应条带强度增加。提示酸性环境不影响SIgA对变形链球菌的识别。Objective To test the salivary immunoglobulin A antibody activity to Streptococcus mutans in normal with in acid environment. Methods Streptococcus mutans strains were isolated from 20 volunteers, serotyped by biochemical test and PCR, and genotyped by AP-PCR. Unstimulated secretions from submandibular glands and sublingual glands were collected from volunteers by modified collectors. Each identified Streptococcus mutans genotype was cultured in two groups: control group was cultured in BHI broth pH7.2 at 37℃ for 2 h; acid shock group were cultured in TYEG broth (pH5.5) at 37℃ for 2 h. Analysis of SIgA activity to Streptococcus mutans genotypes in different groups was detected by Western blot. Results ①TheSIgA of each individual could response to his own Streptococcus mutans strains and the reference strains; ②The same individual had different SIgA activity to different genotype strains;③There were no significant difference between acid groups and control groups, in spite that some bands had strong or weak intensity. Conclusions Although Streptococcus mutans could express acid shock proteins in stress, the present study suggests that these new proteins have no qualitative effect on the reaction of SIgA to Streptococcus mutans.

关 键 词：变形链球菌 酸性环境 分泌型IgA 体外研究 免疫反应 随机引物聚合酶链反应 分泌型免疫球蛋白 Western 特异性结合 唾液SIGA 免疫印迹 酸性条件 不同基因型 PCR) 厌氧培养 凝胶电泳 细胞蛋白 参考菌株 方法分析 blot 标准菌株 

分 类 号：R781.1[医药卫生—口腔医学]