CIK细胞对裸鼠腹膜移植人胃癌细胞增殖和凋亡影响的实验研究  

Study on offect of CIK cells on tumor cells proliferation and apoptosis in peritoneal metastasis model for human gastric carcinoma in nude mice

作　　者：杨进强[1] 李勇[1] 赵群[1] 陈少轩[1] 邝钢[2] 范立侨[1] 宋振川[1] 赵雪峰[1] 王冬[1] 

机构地区：[1]河北医科大学第四医院外三科,河北石家庄050011 [2]河北省肿瘤研究所病理研究室,河北石家庄050011

出　　处：《肿瘤防治杂志》2005年第6期425-428,共4页China Journal of Cancer Prevention and Treatment

摘　　要：目的研究细胞因子诱导的杀伤细胞(Cytokine inducedkiller,CIK)对裸鼠腹膜移植人胃癌细胞增殖和凋亡的影响。方法体外制备CIK细胞。建立人胃癌细胞裸鼠腹膜移植小鼠模型,模型鼠腹腔注射CIK细胞,透射电镜观察腹膜移植癌组织形态学变化,流式细胞术(FCM)检测癌细胞增殖周期中G0/G1、S、G2/M期细胞比率、增殖指数(proliferationIndex,PI)和细胞凋亡指数(apoptosisIndex,AI)的变化。结果模型鼠腹膜癌组织细胞的超微结构出现不同程度的凋亡形态学改变,FCM检测DNA直方图上呈现有凋亡峰。G0/G1期细胞比率为(70.73±2.02)%～(75.88±2.23)%,AI为(7.50±2.69)%～(13.16±2.58)%,与对照组相比显著增加,P=0.0000,P=0.008,P<0.01;S期细胞比率为(14.96±1.32)%～(15.34±1.30)%,无显著变化,P>0.05;G2/M期细胞比率为(14.31±1.51)%～(8.91±0.86)%,PI为(29.27±2.05)%～(24.12±2.10)%,均显著降低,P=0.000、0.008、0.033、0.043,P<0.01。随CIK细胞注射剂量的增加和作用时间延长,G0/G1期细胞比率和AI逐渐增加,而G2/M期细胞比率和PI下降,差异均有统计学意义,P<0.05或P<0.01。细胞AI与G0/G1期细胞比率正相关,r=0.676,与PI负相关,r=-0.659。结论CIK细胞可以影响胃癌腹膜种植转移细胞的细胞周期,并具有诱导细胞凋亡的作用。OBJECTIVE:To study the effect of cytokine-i nduced killer (CIK) on tumor cells proliferation and apoptosis in peritoneal met astasis model for human gastric carcinoma in nude mice.METHODS: Produce CIK cells in vitro. The peritoneal metastasis models for gastric c arcinoma were established by injecting OCUM-2MD3 cells into abdominal cavity of nude mice. CIK cells were injected into the abdominal cavities of the model mice . The morphological changes were observed by electronic microscope. With flow cy tometry equipment, the rate of tumor cells in G_0/G_1、S、G_2/M stage、PI ind ex and the apoptosis index were respectively detected. RESULTS: After injecting CIK cells, more apoptosis phenomenon was founded by electr onic microscope. The rate of tumor cells in G_0/G_1 stage,(~70.73±2.02 ) %-(75.88±2.23)%,and the apoptosis index,(7.50±2.69)%-(13.16±2.58)%,w ere significantly higher, P=0.000 0,P=0.008, P<0.01, but there was not greatly changed in S stage, P>0.05. On the other hand, the rate of tumo r cells in G_2/M stage, (14.31±1.51)%-(8.91±0.86)%, and PI index, (29.27 ±2.05)%-(24.12±2.10)%,were significantly lowed, P<0.01. The CIK cells role increased following the time and the dosage, P=0.000,0.0.008,0.033 ,0.043,P<0.05 or <0.01. The correlation analysis showed that the tumor cell s apoptosis rate had positive correlation with the rate of tumor cells in G_0/G _1 stage, ~r=0.676 ,and had negative correlation with the proliferation index, r=-0.659. CONCLUSIONS: CIK cells may have an ef fect on the tumor cells cycle of the peritoneal metastasis of gastric carcinoma, and induce them apoptosis.[

关 键 词：胃肿瘤 腹膜转移 CIK细胞 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]