整合素连接激酶mRNA在胃癌组织中表达的研究  

Integrin-linked kinase mRNA expression in gastric carcinoma

作　　者：邬麟[1] 杨一新[2] 周均田[1] 

机构地区：[1]湖南省肿瘤医院内科,湖南长沙410013 [2]中南大学湘雅医学院肿瘤研究所,湖南长沙410008

出　　处：《肿瘤防治杂志》2005年第6期439-442,共4页China Journal of Cancer Prevention and Treatment

摘　　要：目的比较整合素连接激酶(in tegric linkedkimase,ILK)mRNA在胃癌组织及正常胃组织中表达的差异,分析ILKmRNA的表达与胃癌的病理类型、侵袭程度、淋巴转移、临床分期之间的关系。方法取胃癌患者手术切除标本液氮、-80℃冰箱保存,TRIzol法提取总RNA,RT PCR扩增ILKmRNA后对扩增产物进行电泳及图象分析。结果癌组织、癌旁组织与相应正常胃组织中ILKmRNA定性表达阳性率分别为93.3%(28/30)、65.0%(13/20)和45.0%(9/20),胃癌组织ILK mRNA表达阳性率高于癌旁组织及正常胃组织,P=0.001。ILKmRNA表达阳性的标本中,其半定量表达均值胃癌组为1.84±0.429,癌旁组为1.01±0.205,正常组为0.73±0.192,两两比较发现差异有统计学意义,P=0.003和0.000。胃癌组织ILK mRNA表达阳性率的高低与病理类型、侵袭程度、淋巴转移及临床分期无关,P=0.446、0.330和1.000。但在ILKmRNA表达阳性的癌组织中,其半定量表达的均值随侵袭程度、淋巴转移及临床分期递增而随之递增,P=0.022和0.000。结论ILKmRNA在胃癌组织、癌旁组织和相应正常胃组织中均有阳性表达,但在癌组织中表达量高于其他组织。胃癌组织中ILKmRNA表达量高低与侵袭程度、淋巴转移和远处转移及临床分期密切相关。高表达的ILKmRNA预示着胃癌的高度恶性、高侵袭性、高转移性?OBJECTIVE:To investigate the difference of In te grin-linked kinase (ILK) mRNA expression in gastric carcinoma tissue and normal gastric tissue and analysis the relationship among the ILK mRNA expression and malignancy type,aggressive degree, nodal metastasis and clinical staging in gas tric carcinoma.METHODS:The operatic resection sample of ga stric carcinoma tissue were studied. Total RNA were extracted with TRIzol. The e xpression of ILK mRNA were using reverse-transcription polymerase chain reactio n (RT-PCR), followed by electrophoresis and image analysis.RESULTS: The ILK mRNA expression positive rate in operatic resection sample in cluding 30 cases gastric carcinoma, 20 cases adjacent and 20 cases corresponding non-neoplastic normal gastric tissue were 93.3%(28/30), 65.0%(13/20) and 45 .0%(9/20) respectively. The difference among gastric carcinoma , adjacent and c orresponding normal gastric tissue have statistical significance, ~P=0.001 ). The quantum expression in ILK mRNA positive samples in gastric carcinoma, a djacent and corresponding normal gastric tissue were 1.84±0.429,1.01±0.20 5 and 0.73±0.192 respectively. The difference between each other had statisti cal significance, P=0.003, 0.000. No correlation was found in the ILK mRNA expression positive rate with malignancy type,aggressive degree, nodal metasta sis and clinical staging in gastric carcinoma, ~P=0.446 , 0.330, 1.000. But the quantum expression of ILK mRNA was strongly correlated with them, P =0.022, 0.000. CONCLUSIONS:We concluded that the express ion of ILK mRNA was higher in gastric carcinoma tissue than in adjacent and corr esponding normal gastric tissue. The quantum expression of ILK mRNA was correlat ed with malignancy type,aggressive degree , nodal metastasis and clinical stagi ng in gastric carcinoma. Strong expression of ILK mRNA indicated higher malignan cy, invasion, metastasis and lower survival of gastric carcinoma. ILK mRNA might be a novel molecular marker for aggressive gastric carcinoma.[

关 键 词：整合素连接激酶 胃肿瘤/外科学 

分 类 号：R735.2[医药卫生—肿瘤]