兔慢性鼻窦炎模型建模方法的比较与优化改良  

Comparison and improvement in the methods of establishing animal model of experimental chronic sinusitis in rabbits

作　　者：林彬[1] 王挥戈[1] 林心强[1] 刘小芬[2] 黄源春[3] 

机构地区：[1]广东省汕头大学医学院第一附属医院耳鼻咽喉头颈外科,515041 [2]广东省汕头大学医学院第一附属医院动物实验室,515041 [3]广东省汕头大学医学院第一附属医院细菌室,515041

出　　处：《中华耳鼻咽喉头颈外科杂志》2005年第5期343-346,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：广东省科技计划项目 (200128135 );广东省医学科学研究基金(1999410)

摘　　要：目的　探讨改进慢性鼻窦炎动物模型的制备方法。方法　将66只新西兰大白兔制成动物模型,随机分成空白对照组(6只)、假手术Ⅰ、Ⅱ组(各10只)、单纯细菌组(10只)、单纯窦口堵塞组(10只)、窦口堵塞+金黄色葡萄球菌组(10只)、窦口不完全堵塞+窦腔留置棉絮组(10只),术后42d取上颌窦黏膜标本分别进行形态学观察及细菌学检查。结果　各实验组建立慢性鼻窦炎模型成功率:窦口堵塞组为80%,窦口堵塞+金黄色葡萄球菌组为100%,窦口不完全堵塞+窦腔留置棉絮组为100%,单纯细菌组、空白对照组及假手术对照组均为0%。感染鼻窦均表现为中重度慢性炎症,培养的细菌以机会致病菌为主。采用窦口堵塞+金黄色葡萄球菌方法并发上颌窦积脓概率高。结论　相对其他建模方法,窦口不完全堵塞+窦腔留置棉絮的建模方法是一种更理想的慢性鼻窦炎动物模型的制备方法。Objective To compare and improve the methods of establishing animal model of experimental chronic sinusitis in rabbits . Methods Sixty-six New Zealand white rabbits were divided into seven groups: control group, sham-operation group Ⅰ, sham-operation group Ⅱ, bacteria inoculation group, ostia-blocked group, ostia-blocked and bacteria inoculation group, incomplete ostium blocked and set-cotton group. The animals were examined by the mehods of histology and bacteriology after 42 days. Results The positive rate of chronic inflammation in ostium blocked group was 80%, inoculated staphylococcus group was 100%, incomplete ostium blocked and set-cotton group was 100%, and the other groups were 0%. All infected sinuses displayed signs of moderate or severe chronic inflammation. The cultivated bacteria were mainly opportunistic pathogens. The probability of having abscess in maxillary sinus was high in ostia-blocked and bacteria inoculation group. Conclusions The method of incomplete ostium blockage and set cotton can establish stable chronic sinusitis model, it is a simple and perfect method.

关 键 词：兔 慢性鼻窦炎 动物模型 建模方法 优化改良 鼻黏膜纤毛 

分 类 号：R-332[医药卫生]