外源性透明质酸酶对人乳腺癌细胞侵袭力及血管形成能力的影响  

Effects of Exogenous Hyaluronidase on Invasive and Angiogenic Capacity of Human Breast Cancer Cells

作　　者：王良[1] 任国胜[1] 涂刚[1] 石远[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《中国普外基础与临床杂志》2005年第3期216-218,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(编号: 30371393)~~

摘　　要：目的　探讨外源性透明质酸酶(hyaluronidase, Hyase)对人乳腺癌细胞ZR 75 30 侵袭潜力及血管形成能力的影响。方法　通过建立体外侵袭模型和应用双室联合培养技术,观察外源性Hyase对ZR 75 30细胞穿透Matrigel(人工基底膜)的能力以及诱导血管内皮细胞形成血管能力的影响。结果　实验组的穿膜细胞数为(70.625±11.64)个,明显高于对照组的(22.125±6.09)个(P<0.01); 实验组的血管管腔数目为(34.5±2.4)条,明显高于对照组的(8.5±1.5)条(P<0.01)。结论　外源性Hyase有促进人乳腺癌细胞侵袭及诱导血管形成的作用。Objective To study the effects of exogenous hyaluronidase on invasive and angiogenic potential of human breast cancer cell line ZR-75-30.Methods There were two groups in the study: the study group (hyaluronidase group) and the control group. The invasive potential and the angiogenic potential of human breast cancer cell ZR-75-30 were detected by the invasive model in vitro and technique of double-chamber co-culture that human breast cancer cell line ZR-75-30 and human umbilicus vein endothelium cell ECV-304 were co-cultured. Results The penetrating number of tumor cell in the study group (70.625±11.64) was significantly higher than that in the control group (22.125±6.09), P <0.01. The tube number from ECV-304 cell induced by ZR-75-30 cell in the study group (34.5±2.4) was significantly higher than that in the control group (8.5±1.5), P <0.01. Conclusion Exogenous hyaluronidase can reinforth the invasive and angiogenic ability of breast cancer cells.

关 键 词：透明质酸酶 乳腺癌 侵袭力 血管形成 外源性 癌细胞 ECM 

分 类 号：R737.9[医药卫生—肿瘤]