血尿激酶型纤溶酶原激活剂mRNA水平表达与乳腺癌及其淋巴结转移的关系  

Study on Relationship Between mRNA of Urokinase-Type Plasminogen Activator and Breast Cancer, Lymph Nodes Metastasis

作　　者：熊俊[1] 张锦辉[1] 郑启昌[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《中国普外基础与临床杂志》2005年第3期241-243,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　研究血尿激酶型纤溶酶原激活剂(uPA)mRNA水平表达与乳腺癌及淋巴结转移的关系及其临床意义。方法　应用荧光定量RT PCR方法,检测60例乳腺良、恶性肿瘤患者血中uPA mRNA水平的表达,分析其与乳腺癌及其淋巴结转移的关系。结果　uPA mRNA表达在18例良性肿瘤患者中16例为阴性,2 例为低表达。在42例乳腺癌患者中,无淋巴结转移的20例中18例为阳性,其中1例为高表达, 5例为中度表达,12 例为低表达,另2例为阴性; 有淋巴结转移的22例均为阳性,其中16 例为高表达,5 例为中度表达,1 例为低表达。乳腺良、恶性肿瘤患者血中uPA mRNA表达差异有显著性意义(P<0.05); 乳腺癌患者中无淋巴结转移者uPA mRNA的表达强度明显低于有淋巴结转移者(P<0.05)。结论　血中uPA mRNA在乳腺癌中的表达明显高于良性肿瘤,其表达强度与乳腺癌淋巴结转移明显相关,可为临床分期及后续治疗提供依据。Objective To investigate the relationship between the expression of urokinase-type plasminogen activator (uPA) mRNA and breast cancer, lymph node metastasis. Methods Sixty patients with breast tumor were selected randomly and the expression of uPA mRNA was detected with RT-PCR. The patients were divided into benign group (18 cases) and malignant group (42 cases) which included 22 cases with lymph node metastasis and 20 cases without lymph node metastasis. The relationship between uPA mRNA expression and breast cancer, lymph node metastasis was analyzed. Results Among these 18 benign tumors, low expression of uPA mRNA was found in 2 cases and the others were negative. While in 42 cases of malignant tumor, uPA mRNA were positive in 22 cases of lymph node metastasis, 16 of which were high expression, 5 of which were moderate expression, and 1 was low expression. uPA mRNA were positive in 18 of 20 cases of nonmetastatic lymph node, 1 of which was high expression, 5 of which were moderate expression and 12 of which were low expression, the other 2 were negative expression. The expression of uPA mRNA had significant difference between benign and malignant tumors ( P <0.05). The expression in lymph node metastasis was much higher than no lymph node metastasis ( P <0.05). Conclusion The expression of uPA mRNA in malignant breast cancer is obviously higher than that in benign breast tumor. The expression tensity of uPA is highly relevant to lymph node metastasis in malignant breast cancer, which can provide evidence for clinical staging and therapy.

关 键 词：尿激酶型 纤溶酶原激活剂 乳腺癌 荧光定量逆转录酶链式反应 基因表达 基因转移 mRNA 淋巴结转移 

分 类 号：R737.9[医药卫生—肿瘤]