乌司他丁对大鼠肝缺血-再灌流损伤的保护作用  

Protective effect of Ulinastatin(UTI)on hepatic ischemia and reperfusion injury in rats

作　　者：林胜璋[1] 郑志强[1] 吴伟军[1] 俞辉军[1] 游涛[1] 陈聪[1] 

机构地区：[1]温州医学院附属第二医院肝胆胰外科,浙江省温州325027

出　　处：《中华急诊医学杂志》2005年第5期388-390,共3页Chinese Journal of Emergency Medicine

摘　　要：目的研究乌司他丁对肝缺血再灌流损伤的保护作用。方法将45只成年雄性SD大鼠随机分成3组:假手术对照组(A组)、肝缺血30min、再灌流90min组(B组)、缺血前30min静脉注射乌司他丁+肝缺血30min、再灌流90min组(C组)。分别测血浆中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、乳酸脱氨酶(LDH)浓度;测定肝组织中髓过氧化物酶(MPO)含量,并取肝组织作光镜及电镜观察。结果再灌流90min时C组的血浆ALT、AST、LDH值及肝组织MPO含量均低于B组(P<0.01),且C组的肝细胞显微、超微结构损害的改变较B组的轻。结论乌司他丁能抑制肝缺血再灌流时中性粒细胞在肝组织中的聚集,从而对大鼠肝缺血再灌流所致肝细胞的结构和功能损伤有保护作用。Objective To study the protective effect of Ulinastain(UTI)on hepatic ischemia and reperfusion injury.Methods Fourty-five healthy male SD rats were randomly devided into three groups,15 animals in each group.Group A served as sham-operation controls,and group B underwent 90 minutes of reperfusion after 30 minutes of ischemia in the liver.UTI was administered to animals in group C,which were reperfused 90 minutes after 30 minutes of ischemia in the liver.Plasma almine aminotransferase(ALT),aspartate aminotrans-ferase(AST),lactate dehydrogenase(LDH)were measured in each group.The activity of myeloperoxidase(MPO)in liver tissue was also determined,and the hepatic tissue was examined under light and electronic microscopies.Results The levels of plasma ALT,AST,LDH,and content of MPO in the liver tissue in group C were significantly decrease,compared with group B(P<0.01).The histologic changes of the liver tissue under light and electronic microscopies was more obvious in group B than in the group C.Conclusion UTI has the ability to inhibit the neutrophil's accumulation druing hepatic tissue ischemia-reperfusion,so protect ischemia-reperfusion injury in rat liver.

关 键 词：缺血-再灌流损伤 乌司他丁 保护作用 鼠肝 天冬氨酸转氨酶 丙氨酸转氨酶 髓过氧化物酶 乳酸脱氨酶 中性粒细胞 肝组织 SD大鼠 静脉注射 电镜观察 结构损害 功能损伤 肝缺血 肝细胞 对照组 B组 C组 ALT AST MPO IDH 血浆 

分 类 号：R575[医药卫生—消化系统]