HSPC016基因重组工程菌发酵研究  

Research on fermentation process of HSPC016 gene recombinant Escherichia coli BL21

作　　者：邹锋[1] 郝飞[1] 宋志强[1] 易勇[2] 郝进[1] 钟华[1] 叶庆佾[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆市皮肤性病研究所 [2]第三军医大学医学检验系临床微生物及免疫学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第9期904-906,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 2 0 0 2 4 9)~~

摘　　要：目的　研究人毛乳头细胞HSPC0 16基因重组工程菌的发酵工艺。方法　采用德国B Brau公司C 10型5L发酵罐批次发酵,通过对能影响工程菌生长和目的蛋白表达的条件如不同培养基、不同葡萄糖浓度、营养物补充、pH及溶氧调节等进行优化,确定合适的培养条件和发酵工艺。结果　在优化条件下,菌体单产可达45 g/L ,目的蛋白表达量约占总蛋白的18%。结论　此发酵工艺可以提高人毛乳头细胞HSPC0 16/pET 2 8a(+ )基因重组工程菌的收获和目的蛋白表达。Objective To develop a better fermentation process of the HSPC016 gene of human dermal papilla cell recombinant Escherichia coli BL21. Methods The expression of target protein was analyzed to optimize the composition of the medium, the range of pH values, induction time and other factors in the process of fermentation. Results Under the established condition, 45 g of wet bacteria per liter was obtained and the derivative of target protein was about 18% of total protein in host. Conclusion The established fermentative technique enhanced the collection efficiency and expression of target protein.

关 键 词：人毛乳头细胞 HSPC016基因 发酵 大肠杆菌 

分 类 号：Q93-335[生物学—微生物学] R322.99[医药卫生—人体解剖和组织胚胎学]