血吸虫病门静脉高压兔肺组织病变的实验研究  

The experimental study on pathological changes of pulmonary tissues in portal hypertensive rabbits with schistosomal cirrhosis

作　　者：周永贵[1] 杨镇[1] 李岽健[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科,武汉430030

出　　处：《中华外科杂志》2005年第9期587-590,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目(30170920)

摘　　要：目的探讨血吸虫病肝硬化门静脉高压症时肺组织的病理变化和内皮素1(ET1)、NO在肺血管病变机制中的作用。方法运用腹部敷贴法感染血吸虫尾蚴制作大耳白兔肝硬化门静脉高压症动物模型(n=10),采用免疫组化技术和HE染色、Masson三色染色及透射电镜方法研究血吸虫病肝硬化门脉高压症兔肺组织病理改变和ET1、NO合酶(NOS)的表达、分布。结果门脉高压症兔肺组织中ET1、NOS阳性或强阳性表达伴病理学改变,正常兔为阴性或弱阳性,图像定量分析两组灰度值和吸光度值差异具有统计学意义(P<0.01)。结论血吸虫病肝硬化门脉高压症时兔肺组织发生明显的病理改变,ET1、NO可能在血吸虫病门脉高压症肺血管病变发病机制中具有重要意义。Objective To investigate the pathologic features of the pulmonary tissue in portal hypertensive rabbits with schistosomal cirrhosis, and to study the role of endothelin-1 (ET-1) and nitric oxide (NO) in pathogenesis of portal hypertensive pulmonary vasculopathy. Methods The experimental group included 10 rabbits infected percutaneously with cercariae of Schistosomiasis Japonica. The control group included 10 normal rabbits. HE stain, Masson trichrome stain and transmission electron microscopy were applied to detect the pathologic features of the pulmonary tissue. The expression and distribution of endothelin-1(ET-1) and nitric oxide synthase (NOS) in the lung tissues were analyzed by immunohistochemistry. Results After 120 d, the pathological morphology alteration of the pulmonary tissue was observed in the rabbits in experimental group . Both of ET-1 and NOS-containing cells were more abundant in distribution and expression in the lung tissue of experimental group than those of the control group. There was significant difference between the two groups in the parameter of area, lightness and gray level of ET-1 and NOS (P<0.01). Conclusions Pulmonary pathologic changes occur in the portal hypertensive rabbits with schistosomal cirrhosis. ET-1 and NOS-containing cells are more abundant in pulmonary vessel of portal hypertention, then followed by dilation of intrapulmonary vessel. It is deduced that ET-1 and NO might play an important role in the pathogenesis of portal hypertensive pulmonary vasculopathy.

关 键 词：血吸虫病 肺组织病变 实验研究 肝硬化门静脉高压症 肝硬化门脉高压症 MASSON三色染色 肺组织病理改变 免疫组化技术 图像定量分析 ET-1 血吸虫尾蚴 病理学改变 肺血管病变 病理变化 病变机制 动物模型 大耳白兔 HE染色 

分 类 号：R575.2[医药卫生—消化系统]