神经鞘糖脂生物合成抑制剂D-PDMP对神经干细胞增殖、分化能力的影响  

Effects of inhibitors of glycosphingolipid biosynthesis D-PDMP on proliferation and differentiation of neural stem cells.

作　　者：林玲[1] 林建银[1] 郑志竑[1] 江琼[1] 胡建石[1] 

机构地区：[1]福建医科大学分子医学研究中心,福建福州350004

出　　处：《中国药理学通报》2005年第5期591-594,共4页Chinese Pharmacological Bulletin

基　　金：福建省科技计划重大资助项目(No2001Z020)

摘　　要：目的探讨神经鞘糖脂生物合成抑制剂DPDMP对神经干细胞增殖和分化能力的影响。方法用不同浓度的DPDMP处理体外培养的神经干细胞,通过神经球直径大小和数量、细胞计数、MTT分析和诱导分化实验,评价其对神经干细胞增殖和分化能力的影响。结果经DPDMP处理后培养的神经球减小,细胞计数和MTT分析都表明细胞增殖能力明显受到抑制,较高DPDMP浓度可导致神经干细胞死亡;神经干细胞的诱导分化能力也明显减弱,分化的细胞数较少,但仍可诱导成神经元和星形胶质细胞。结论神经鞘糖脂生物合成抑制剂DPDMP能抑制体外培养的神经干细胞增殖,甚至导致细胞死亡,并可减弱神经干细胞的诱导分化能力。Aim To explore the effects of inhibitors of glycosphingolipid biosynthesis D-PDMP on the proliferation and differentiation of neural stem cells. Methods Neural stem cells cultured in vitro were treated with different concentration of D-PDMP. The effects of D-PDMP on proliferation and differentiation of neural stem cells were evaluated by counting of neurospheres size and cellular number, MTT assaying and the experiment of inducing differentiation.Results Neurospheres were smaller in size after treated with D-PDMP. The data of cellular counting and MTT assay suggested that D-PDMP could inhibit proliferation of neural stem cells and lead cells death in higher concentration, D-PDMP also reduced the differentiation ability of neural stem cells. Although differentiated cells were fewer, they could be induced into neurons and astrocytes.Conclusion The inhibitors of glycosphingolipid biosynthesis D-PDMP could inhibit the proliferation of neural stem cells, even lead cells death. It also reduced the differentiation ability of neural stem cells.

关 键 词：神经干细胞 D-PDMP 增殖 分化 

分 类 号：R-332[医药卫生] R322.81