延胡索乙素对豚鼠单个心室肌细胞钾离子通道的影响  

Effects of dl-tetrahydropalmatine on potassium current in isolated myocytes of guinea pig

作　　者：刘玉梅[1] 周宇宏[1] 单宏丽[1] 冯铁明[1] 乔国芬[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室

出　　处：《中国药理学通报》2005年第5期599-601,共3页Chinese Pharmacological Bulletin

摘　　要：目的研究延胡索乙素(dltetrahydropalmatine,THP)对正常豚鼠心室肌细胞钾电流的影响,旨在探讨延胡索乙素抗心律失常作用机制。方法酶解法分离豚鼠单个心室肌细胞,应用全细胞膜片钳技术记录延胡索乙素对豚鼠单个心室肌细胞钾电流的影响。结果延胡索乙素可明显抑制延迟整流钾电流(IK)和内向整流钾电流(IK1),并呈剂量依赖性。结论延胡索乙素抗心律失常作用机制可能与它对心肌细胞钾通道作用有关,THP可抑制IK和IK1,使动作电位时程(APD)和有效不应期(ERP)延长从而发挥其抗心律失常作用。Aim To investigate the antiarrhythmic mechanism of dl-tetrahydropalmatine (THP) on the potassium current in guinea pig′s ventricular myocytes. Methods Enzyme digestion was used to isolate single ventricular myocyte and whole cell patch clamp technique was used to record potassium current. Results In ventricular myocytes of guinea pig, the delayed rectified potassium current(I_K) and the inwardly rectifying potassium current (I_ K1) were blocked by THP in a concentration-dependent fashion. Conclusion The mechanism of anti-arrhythmic action of THP may be relative to potassium current, THP inhibited I_K and I_ K1 to prolong the action potential duration (APD) and effective refractory period (ERP).

关 键 词：延胡索乙素 心肌细胞 膜片钳 

分 类 号：R-332[医药卫生] R282.71