大鼠C6脑胶质瘤模型的病理特征与MRI的观察  

Observation of pathological characteristics in C6 rat brain glioma model and its MRI images

作　　者：李明[1] 冯华[1] 李飞[1] 王宪荣[1] 吴南[1] 陈志[1] 吴国材[1] 卢佳友[1] 林江凯[1] 

机构地区：[1]第三军医大学附属西南医院神经外科,重庆400038

出　　处：《中华神经外科杂志》2005年第5期279-282,共4页Chinese Journal of Neurosurgery

摘　　要：目的建立SD大鼠C6胶质瘤模型并对其病理特征及MRI进行观察。方法50只SD大鼠随机分成5组,每组10只,C6胶质瘤细胞悬液立体定向接种于大鼠的右侧尾状核,接种后观察大鼠的生活状态、生存期;分别于接种后不同时段进行MRI观察肿瘤生长特性及肿瘤体积的测量;取不同时段组大鼠脑标本行脑组织HE染色、透射电镜(transmissionelectronmicroscope,TEM)、脑组织含水量测量(与10只正常大鼠对照)、胶质纤维酸性蛋白(GFAP)免疫组化检查。结果立体定向颅内接种成功率97.5%,未见远处及颅外转移,肿瘤在一定时期内生长较快,脑水肿随肿瘤的生长明显加重,生存期观察组中7只荷瘤鼠死亡,3只肿瘤自发部分消退。结论立体定向建立大鼠C6胶质瘤模型成功率高,接种后颅内肿瘤呈浸润性生长,与人脑胶质瘤具有相似性,由于生存期观察组中有部分荷瘤鼠出现肿瘤自发部分消退,故应用该模型评价治疗效果时应慎重;T1WI增强扫描可清晰显示肿瘤影像,且能更早发现肿瘤;MRI联合病理可较好反映肿瘤生长方式及发展过程。Objective To establish C6 glioma model in rat brain and to study its pathological characteristics and MRI images.Methods There were 50 SD rats classified into five groups in random.The suspension of cells was stereotaxically injected into the right caudate.The examinations such as MRI, HE staining, transmission electron microscope(TEM)brian water(contrast to normal rat brain), immunohistochemical staining of GFAP were performed at different time points after implantation.Results There was significance difference in statistics between brain water of C6 glioma rats at different time points and that of normal rats. The MRI images and the histological sections showed a marked increase in size with focal mass effect,perifocal edema.The examination of transmission electron microscope showed that C6 tumor had high invasiveness.The success rate of stereotactic implantation was 97.5%.There were three rats whose tumors spontaneously regressed partly in the survival time examination group and no extracranial metastasis after implantation. Conclusions The rat C6 brain tumor model was successfully established and it mimicked the human tumor with respect to its development, progression,invasion.It should be very careful to evaluate curative effect of the model because part of C6 tumor spontaneously regressed.The tumor images could be displayed vividly and discoveryed in early stages.The growth mode and development of C6 glioma could be reflected in C6 glioma MRI images associated with its pathological characteristics.

关 键 词：胶质瘤模型 MRI 胶质纤维酸性蛋白 立体定向 脑组织含水量 免疫组化检查 肿瘤生长方式 SD大鼠 不同时段 病理特征及 c6胶质瘤 接种成功率 浸润性生长 人脑胶质瘤 接种后 生存期 细胞悬液 生活状态 肿瘤体积 生长特性 HE染色 

分 类 号：R739.41[医药卫生—肿瘤]