WRKY转录因子及其在植物防御反应中的作用  

WRKY Transcription Factors and Their Roles in Plant Defense Responses

作　　者：李蕾[1] 谢丙炎[1] 戴小枫[2] 杨宇红[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《分子植物育种》2005年第3期401-408,共8页Molecular Plant Breeding

基　　金：国家重大基础研究规划973“农林危险生物入侵机理与控制基础研究”项目第一课题资助内容;国家自然科学基金项目(30270236)。

摘　　要：WRKY蛋白是只存在于植物中较为复杂的转录因子家族。由WRKY蛋白N端高度保守的WRKYGQK氨基酸序列及其特殊的锌指结构而命名的WRKY结构域能专性与其顺式作用元件W-盒[(T)(T)TGAC(C/T)]结合。根据WRKY结构域的数量及锌指结构的特征将WRKY蛋白家族分为3类,其中拟南芥中第三类家族中几乎所有的WRKY成员都与应答生物胁迫有关。WRKY蛋白的表达特性为快速、瞬时、具组织特异性诱导表达。目前,WRKY蛋白被广泛认为参与植物生物与非生物胁迫应答反应、植物衰亡、种皮及毛状体发育及果实发育等一系列的生理活动,本文综述了WRKY转录因子的结构特征及其功能特性,重点讨论了它们在植物防卫反应中的调控作用。The WRKY proteins comprise a superfamily of transcription factors which are unique to plants. The WRKY domain defined by the conserved amino acid seque nse WRKYGQK at its N-terminal end and a novel zinc-finger-like motif is able to bind specifically to the DNA sequence motif(T)(T)TGAC(C/T), which is known as th e cis-elements W-box to WRKY proteins. The WRKY proteins are devided into three groups on the basis of both the number of WRKY domains and the features of their zinc-finger-like motif. In Arabidopsis, nearly all group-Ⅲ members respond to diverse biotic stresses. The expression behavior of induced WRKY genes are often extremely rapid, transient and tissue-specific. Recently, family members appear to be involved in the regulation of various physiological programs, including b iotic or abiotic stresses responses, leaf senescence, trichome development, embr yogenesis and seed development in plants. This review elucidates the structure f eatures and functional characteristics of the WRKY transcription factors, and ba sed on current reseach, summarises the regulatory roles of transcription factors in plant defense responses.

关 键 词：转录因子 植物防御反应 顺式作用元件 植物防卫反应 锌指结构 氨基酸序列 组织特异性 非生物胁迫 表达特性 诱导表达 应答反应 生理活动 果实发育 功能特性 结构特征 调控作用 结构域 蛋白 家族 拟南芥 毛状体 种皮 

分 类 号：Q946.1[生物学—植物学]