观察原代培养新生鼠脊髓神经元中溶酶体的变化与影响  

Changes and effect of lysosome in primary cultured newborn rat spinal neurons

作　　者：于雁玲[1] 王敏 王春芳[3] 

机构地区：[1]山西省儿童医院医务科,030013 [2]山东省莱钢集团淄博锚链有限公司医院内科 [3]山西医科大学医学实验中心分子生物学教研室

出　　处：《中国药物与临床》2004年第4期261-263,共3页Chinese Remedies & Clinics

基　　金：山西医科大学校长基金资助项目(SY20020617)

摘　　要：目的探讨培养脊髓神经元中溶酶体的影响因素。方法原代分离细胞培养大鼠脊髓神经元,培养细胞荧光染色,酶组织化学染色。结果培养的脊髓神经元随培养天数的增加,神经元的突起延长,且突起间逐渐相互连接。神经元中溶酶体经酸性磷酸酶(ACPase)染色后可见散在分布于神经元的胞体及突起内,细胞核ACPase反应阴性。经吞噬荧光黄(luciferyellow,LY)证实,培养的脊髓神经元在施加佛波醇肉豆蔻酸乙酯(PMA)后可见脊髓神经元中胞体及突起均有荧光,且荧光呈网络状分布,胞核无荧光。说明PMA有利于胞体及突起的生长。结论培养脊髓神经元中,溶酶体存在于胞体及突起中。Objective To investigate the effects of lysosome in spinal cultured neurons.Methods Primary culture of spinal neurons was made from newborn Wistar rats;enzyme histochemical technique and fluorescence staining were used.Results Along with the culture time the spinal neuron increased in number,neuron process became lengthened,and a connection of processes formed gradually.Lysosome in neurons via ACPase staining was seen to disperse in neuron's soma and process; the nucleus showed a negative reaction to ACPase .Lucifer yellow (LY) absorption revealed that the cultured neurons which had been treated by phorbol myristate acetate(PMA) showed fluorescence in their soma and process, the fluorescene occurred as reticulated, but did not occur in nuclei.This suggested PMA benefit the growth of soma and process. Conclusion In cultured spinal neurons, lysosomes exsited in cytoplasm and processes.PMA could change the shape of cultured spinal neurons and the distribution of lysosomes.

关 键 词：脊髓神经元 溶酶体 原代培养 新生鼠 酶组织化学染色 酸性磷酸酶 细胞培养 原代分离 荧光染色 培养细胞 肉豆蔻酸 PMA 突起 胞体 细胞核 分布 荧光黄 可见 

分 类 号：R741[医药卫生—神经病学与精神病学]