水牛牛巴贝斯虫体外培养的研究Ⅱ．冷冻血清的应用和筛选  

Contineous In vitro Cultyvation of Babesia bovis Collectedfrom Infected BuffaloⅡ.Screening and Use of Frozen Serum

作　　者：赵俊龙[1] 姚宝安[1] 马丽华[1] 刘钟灵[1] 

机构地区：[1]华中农业大学

出　　处：《中国兽医学报》1994年第2期118-120,共3页Chinese Journal of Veterinary Science

基　　金：国家八五科技攻关课题

摘　　要：应用冷冻血清对１株采自自然感染的水牛牛巴贝斯虫进行了长达７２ｄ的体外连续培养，共继代２０次，培养７２ｈ红细胞染虫率最高达１４．１％，平均为８％～１０％。培养２０ｄ和３０ｄ的牛巴贝斯虫经液氮保存复苏后，接种于去脾水牛犊均引发了严重的牛巴贝斯虫病，从而说明已建立了水牛牛巴贝斯虫的体外连续培养，且经培养后的牛巴贝斯虫致病力没有改变。本试验利用６头份的冷冻健康水牛血清同时进行培养，结果发现，并非所有的健康水牛血清均适合于体外培养牛巴贝斯虫。Babesia bovis collected from a naturally infected buffalo was cultured contineously in artificial medium for 72 days, during which the culture was renewed 20 times. The maximum percentage of parasitized erythrocytes(PPE ) with the parasites cultured for 72 hours was 14.1%, the average 8%to 10%. The inoculation experiment of the spleenectomized buffalo calves with the frozen in liquid nitrogen and resuscitated subcultures of 20 and 30 days resulted in severe babesiasis. It was shown that the conti-neous culture ofBabesia bovishas been establishe and that its virulence did not attenu-ate. Screening assay indicated that not all the frozen sera of healthy buffaloes used to repare the culture medium were suitable for in vitro cultivation of Babesia bovis.

关 键 词：血清 牛巴贝斯虫 体外培养 水牛 

分 类 号：S852.722[农业科学—基础兽医学]