慢性应激对大鼠海马Bcl-xl表达的影响及应激后的变化  

Effects of Chronic Stress on the Expression of Bcl-xl in Hippocampus of the Rats

作　　者：毕波[1] 王哲[1] 彭淼[1] 金魁和[1] 韩继阳[1] 朱宇章[1] 

机构地区：[1]中国医科大学精神医学与医学心理学教研室,辽宁沈阳110001

出　　处：《中国临床心理学杂志》2005年第2期144-146,共3页Chinese Journal of Clinical Psychology

摘　　要：目的:探讨慢性应激对大鼠海马神经元Bcl-xl蛋白表达的影响及其应激后的变化。方法:采用慢性强迫冰水游泳制作动物模型。运用open-field法观察大鼠行为学的变化,运用免疫组织化学方法观察大鼠海马DG区、CA3区Bcl-xl的变化。结果:与对照组相比,实验组1大鼠海马CA3区齿状回(DG)区Bcl-xl平均灰度值显著增加(t=4.69,P<0.05和t=3.77,P<0.01),实验组2平均灰度值与对照组2相比同样增加(t=3.35,P<0.05和t=3.30,P<0.05)。结论:慢性应激使大鼠海马Bcl-xl表达降低,应激三十天后,其表达仍低于对照组。Objective: To observe the changes of Bcl-xl expression in hippocampus of the rats with the forced-swimming and the changes of Bcl-xl after poststress. Methods: The protocol was established with the forced-swimming as the chronic stress model. Open-field test was executed to measure the behaviors of the rats. Immunohistochemistry was used to observe the changes of Bcl-xl expression in the hippocampus. Results: Compared to the control group,the expression of Bcl-xl in CA3 region of the hippocampus and dendate gyrus(DG)of the rats was decreased morphologically. With the computerized image analysis, the gray degree increased significantly (P <0.01) and the changes showed the same results after the poststress. Conclusion: Chronic stress decreased the expression of the Bcl-xl in CA3 region and DG in the hippocampus of the rats and the changes showed the same results after the poststress.

关 键 词：BCL-XL表达 大鼠海马 慢性应激 免疫组织化学方法 平均灰度值 海马CA3区 海马神经元 对照组 蛋白表达 动物模型 0.05 表达降低 实验组 行为学 齿状回 相比 

分 类 号：R737.25[医药卫生—肿瘤] R965.2[医药卫生—临床医学]