噬菌体展示TM-TNF-α模拟肽体内杀瘤效应的研究  

The anti-tumor effects of TM-TNF-α mimic peptide displayed on phages in vivo

作　　者：熊霞辉[1] 喻明霞[1] 石文芳[1] 李清芬[1] 林莉[1] 冯玮[1] 徐勇[1] 李卓娅[1] 

机构地区：[1]华中科技大学同济医学院免疫学系,武汉430030

出　　处：《中华微生物学和免疫学杂志》2005年第4期282-285,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家高科技研究发展计划 (863计划 ;2 0 0 1AA2 15 43 1)资助项目

摘　　要：目的　研究噬菌体展示TM -TNF- α模拟肽的体内杀瘤效应及机制。方法　大量制备所需的噬菌体展示肽,比较不同展示肽对小鼠的肝癌细胞H2 2的体外杀瘤效应,挑取杀瘤效应最好的展示肽并选择其合适的滴度进行体内实验。小鼠接种H2 2细胞3d后,于肿瘤接种部位皮下注射噬菌体展示肽,观察肿瘤的生长情况。结果　噬菌体展示TM- TNF -α模拟肽在体内能明显抑制肿瘤的生长(P <0 .0 1)。且抑瘤率与展示肽之间呈剂量依赖关系。HE染色发现展示肽治疗组肿瘤组织中有大量淋巴细胞浸润,而sTNF- α治疗组除淋巴细胞浸润还可见中性粒细胞、浆细胞浸润。结论　直接注射噬菌体展示TM -TNF -α模拟肽可在体内有效杀瘤,其杀瘤机制可能不同于分泌型TNF -α。Objective To study the anti-tumor effects of TM-TNF-α mimic peptide displayed on phages in vivo. Methods Peptides-presenting phages were prepared and their anti-tumor effects were compared. Then, the peptide with the best effect and the suitable concentration was subcutaneously injected on day 3 after H22 tumor cell challenge. The tumor growth was observed. Results TM-TNF-α mimic peptide displayed on phages can dramatically inhibit the growth of tumor (P<0.01) in a dose dependent manner. HE staining showed infiltration of lymphocytes in the tumor tissues, while there was infiltration of neutrophils and plasma cells but not lymphocytes in the tumor tissues treated by s-TNF. Conclusion Direct injection of TM-TNF-α mimic peptide displayed on phages could effectively suppress the tumor growth. The mechanism involved in the tumoricidal activity induced by peptides was different from that of sTNF-α.

关 键 词：TM-TNF-Α 噬菌体展示 杀瘤效应 模拟肽 分泌型TNF-Α 淋巴细胞浸润 肝癌细胞H22 剂量依赖关系 H22细胞 中性粒细胞 浆细胞浸润 体内实验 皮下注射 接种部位 抑制肿瘤 肿瘤组织 HE染色 直接注射 杀瘤机制 治疗组 抑瘤率 

分 类 号：R73-3[医药卫生—肿瘤]