T_H细胞在记忆性CTL介导的肿瘤抑制中的作用研究  

The role of T_H cells in memory CTL mediated tumor protection

作　　者：高丰光[1] Germain J. P. Fernando 刘文军 

机构地区：[1]厦门大学医学院免疫学教研室,361005 [2]entre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane QLD,4102, Australia [3]Clinical Medical Virology Centre, University of Queensland, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Brisbane QLD,4029, Australia

出　　处：《中华微生物学和免疫学杂志》2005年第4期286-291,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的　观察记忆性CTL的抑瘤作用是否需要抗原特异性TH 细胞的辅助。方法　表达OVAT细胞表位SIINFEKL特异性TCR的T细胞转输RAG- - 小鼠,经相应T细胞表位多肽刺激后产生记忆性CTL ,将此记忆性CTL转输已产生特异性和非特异性TH 细胞的C5 7BL 6小鼠体内并接种肿瘤细胞EG7。结果　经T细胞表位多肽免疫后,SIINFEKL特异性TCRT细胞成功在RAG- - 小鼠体内增殖并分化为记忆性CTL ;抗原特异性TH 细胞可辅助产生较多效应性CTL但并没有完全的肿瘤抑制作用;记忆性CTL的完全抑瘤活性需要抗原特异性TH 细胞的辅助。结论　机体对肿瘤的长期完全杀伤作用不但需要肿瘤特异性抗原诱导产生的记忆性CTL ,而且需要特异性TH 细胞的辅助,肿瘤疫苗的设计必须包括特异性的CTL表位多肽和辅助性TH 细胞多肽。Objective To study the role of T H cell in the activation of memory T cells and shifting to functional tumor killer cells. Methods Adoptive transfers SIINFEKL specific CTL to RAG -/- mice and then immunized them with SIINFEKL to generate ovalbumin specific memory CTL. C57BL/6 mice were adoptive transferred with memory CTL immunized with T-helper epitopes of ovalbumin and then challenged with ovalbumin specific tumor cells. Results With SIINFEKL immunization, specific CTL proliferated and acquired memory CTL phenotypes; OVA specific T H cells could help the host to generate more specific CTL but this effect is not strong enough to get tumor inhibition. Memory CTL needs T H cells to completely regress the development of tumor formation. Conclusion Long lasting antitumor effect needs both antigen specific memory CTL and specific T H cells and so the design of tumor vaccine should include specific CTL and T H peptides.

关 键 词：TH细胞 CTL 记忆性 作用研究 C57BL/6小鼠 抗原特异性 介导 表位多肽 肿瘤抑制作用 T细胞表位 肿瘤特异性 抑瘤作用 肿瘤细胞 非特异性 体内增殖 多效应性 抗原诱导 杀伤作用 肿瘤疫苗 TCR 免疫后 性需要 辅助性 

分 类 号：R73-3[医药卫生—肿瘤]