单纯疱疹病毒特异性糖蛋白抗原的原核表达及纯化  

Prokaryotic Expression and Purification of the Specific Glycoprotein Antigens of Herpes Simplex Virus

作　　者：孙萍[1] 杨军[1] 李保昌[1] 王全立[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《生物技术通讯》2005年第3期265-267,共3页Letters in Biotechnology

摘　　要：原核表达并纯化单纯疱疹病毒特异性糖蛋白抗原,用于建立单纯疱疹病毒的临床检测方法。选择单纯疱疹病毒特异性糖蛋白型共同抗原gD以及型特异性抗原gG1(Ⅰ型)、gG2(Ⅱ型),合成PCR引物,分别扩增其DNA片段,插入原核表达载体pQE30,转化大肠杆菌M15,筛选高表达菌株,进行初步纯化鉴定。结果获得了表达菌株,对高表达的gD初步进行了包涵体的复性及纯化,为建立单纯疱疹病毒临床检测方法奠定了基础。To express and purify the specific glycoprotein antigens of herpes simplex virus (HSV) as used in HSV clinical detection. HSV type-common glycoprotein gD gene and type-specific glycoproteins gG1(typeⅠ) and gG2 (typeⅡ) genes amplified by PCR were subcloned into prokaryotic vector pQE30 and expressed in E.coli M15. HSV glycoprotein gD was highly expressed and purified from the inclusion body. HSV specific glycoproteins were expressed successfully in prokaryotic system therefore provides the basis for further research on HSV clinical detection.

关 键 词：单纯疱疹病毒 蛋白抗原 原核表达载体 检测方法 特异性抗原 PCR引物 DNA片段 共同抗原 大肠杆菌 纯化鉴定 高表达 糖蛋白 M15 包涵体 临床 gD 菌株 

分 类 号：Q78[生物学—分子生物学] S858.31[农业科学—临床兽医学]