PDGF和EGF对牙周膜细胞增殖、纤维结合蛋白和碱性磷酸酶活性的影响  

Effect of Platelet-Derived Growth Factor-BB and Epidermal Growth Fact or on the Proliferation, Fibronectin and Alkaline Phosphatase Activity of Human Periodontal Ligament Cells

作　　者：吉建新[1] 廖伟娇[2] 邱志辉[3] 潘广祠[1] 张婷[2] 

机构地区：[1]广州医学院第一附属医院口腔科,广州510120 [2]广州医学院第一附属医院检验科,广州510120 [3]广州呼吸疾病研究所,广州510120

出　　处：《热带医学杂志》2005年第3期267-270,281,共5页Journal of Tropical Medicine

基　　金：广州市科技局资助项目(No.1999-J-008-01)

摘　　要：目的探讨血小板衍生生长因子-BB(PDGF-BB)和表皮生长因子(EGF)对人牙周膜(PDL)细胞增殖、纤维结合蛋白(Fn)和碱性磷酸酶(APLase)活性的影响。方法体外培养人PDL细胞,与不同浓度的PDGF-BB、EGF或PDGF-BB+EGF作用,用噻唑盐(MTT)法观察PDL细胞增殖的情况,ELISA法检测Fn水平,用酶动力学方法检测ALPase的活性。结果PDGF-BB明显促进PDL细胞增殖,在1～50ng/ml和5d范围内,呈浓度-时间依赖关系,最佳效应时间和浓度为3d和10ng/ml,比对照组增加了256.1%(P<0.001),但对PDL细胞Fn水平无明显影响(P>0.05),却显著抑制PDL细胞的ALPase活性(P<0.01)。低浓度的EGF对PDL细胞的增殖没有影响(P>0.05),(10～50)ng/ml的EGF从第3天起,可促进PDL细胞的增殖,差异显著(P<0.05),但10ng/ml与50ng/ml之间无显著性差异(P>0.05),其最佳效应浓度为10ng/ml,最大效应在用药后4d,此时与对照组相比增加了124.1%,EGF可使PDL细胞Fn水平明显提高(P<0.01),但抑制ALPase的活性(P<0.001)。PDGF-BB和EGF二者联合应用,在促进增殖、提高Fn的水平和抑制ALPase活性方面均具有协同效应。结论PDGF-BB、EGF均可促进人PDL细胞的增殖、抑制PDL细胞的ALPase活性,但PDGF-BB对其Fn水平没有明显影响,而EGF可使其Fn水平明显提高,二者联合具有协同效应。Objective To evaluate the in vitro biological effects of plat elet-derived growth factor-BB(PDGF-BB) and/or epidermal growth factor(EGF) on th e proliferation, fibronectin and alkaline phosphatase activity of human periodon tal ligament(PDL) cells. Methods PDL cells were obtained from healthy donors. C ells(6×105/200 μL) were cultured for 1,2,3,4, or 5 days in DMEM supplemented w ith 2% FBS alone(control),or containing PDGF-BB(1,5,10 or 50 ng/ml), or containi ng EGF(1,5,10 or 50 ng/ml) or containing both. Proliferation of the PDL cells wa s measured by MTT colorimeteric assay. Fibronectin was determined by ELISA. ALPa se activity was measured by enzyme kinetic methods. Data was analyzed by ANOVA. Results PDGF-BB induced proliferation of PDL in a dose-and time dependent manne r(P<0.01) with an optimal concentration of 10ng/ml, at day 3. Maximal effect on proliferation was 256.1%. PDGF-BB inhibited ALPase activity but did not affect f ibronectin synthesis. EGF up-regulated the synthesis of fibronectin and inhibite d ALPase activity. Lower concentration of EGF demonstrated no significant differ ences from control, whereas it enhanced the proliferation of PDL cells at 10～50 ng/ml, with an optimal concentration of 10ng/ml, at day 4. Maximal effect on pro liferation was 124.1%. The effect of combination of PDGF-BB with EGF was the mos t signification one (P<0.001). Conclusion PDGF-BB and/or EGF can promote the pr oliferation of PDL cells and inhibited ALPase activity. EGF up-regulated the syn thesis of fibronectin, but not the PDGF. The data suggested that these growth fa ctors might be valuable in promoting the regeneration of periodontal connective tissue.

关 键 词：牙周膜细胞 血小板衍生生长因子-BB 表皮生长因子 

分 类 号：R781.4[医药卫生—口腔医学]