硒对异丙肾上腺素致损伤大鼠心肌SCN5A基因表达的影响  

Effects of selenium on the expression of SCN5A in myocardium of rats treated with Isoproterenol

作　　者：李凤兰[1] 李晖[1] 范启明[1] 杨宝峰[1] 于卓[1] 陈诗慧[1] 金剑峰[1] 马宁[1] 

机构地区：[1]哈尔滨医科大学生物化学与分子生物学教研室,150086

出　　处：《中国地方病学杂志》2005年第3期285-287,共3页Chinese Jouranl of Endemiology

基　　金：黑龙江省教育厅海外学人科研资助项目(1054QH014);黑龙江教育厅科技资助项目(10553040)

摘　　要：目的研究异丙肾上腺素(ISP)致大鼠心肌损伤模型中,编码电压门控Na+通道α亚单位基因SCN5A表达的变化,以及硒对其表达的影响。方法24只Wistar大鼠雌雄各半,随机分为3组,即对照组、ISP组、硒+ISP组。测定心肌丙二醛(MDA)的水平;采用逆转录聚合酶链反应(RT-PCR)和Western-blot方法检测心肌SCN5A基因的表达变化。结果与ISP组比较,硒+ISP组MDA显著降低(P<0.05),但高于对照组(P<0.05);ISP组SCN5A基因mRNA和蛋白的表达均较对照组显著增加,而硒+ISP组降低其表达。结论异丙肾上腺素可使心肌SCN5A基因mRNA和蛋白的表达显著增高;硒对异丙肾上腺素致大鼠心肌损伤有保护作用并能逆转ISP引起的SCN5A基因表达的改变。Objective To study the expression of rat cardiac Na+ channel gene(SCN5A) and the protective effects of selenium in ISP myocardial damage mode l. Methods Twenty-four Wistar rats were randomly divided into three groups: c ontrol group, ISP group, selenium group. The content of MDA was measured in myoc ardium. The expression of SCN5A was detected by means of reverse transcription p olymerase chain reaction(RT-PCR) and Western blot. Results Compared with ISP g roup, the content of MDA in myocardium was decreased significantly in selenium g roup, whereas was higher than that in control group (P < 0.05). Upregulation of SCN5A was observed in ISP group and the expression was decreased in selenium gro up(P < 0.05). Conclusions The results suggested that the expression of SCN5A was increased significantly in rat myocardium due to ISP. Selenium had a protect ive effect in ISP myocardial damage model and reversed the upregulation of SCN5A expression induced by ISP.

关 键 词：异丙肾上腺素 亚硒酸钠 硒 大鼠 心肌 SCN5A 基因表达 心肌细胞 

分 类 号：Q786[生物学—分子生物学]