自体颈动脉体细胞团块纹状体内移植对慢性帕金森病猴行为学变化的影响  

Effect of intrastriatal autograft of carotid body cell aggregates on behavior rehabilitation in monkeys with chronic Parkinson disease

作　　者：张允建[1] 曹学兵[1] 贾清[2] 朱清[1] 廖晓峰[3] 杨正明[4] 孙圣刚[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,湖北省武汉市430030 [2]华中科技大学同济医学院附属协和医院放射科,湖北省武汉市430030 [3]华中科技大学同济医学院附属协和医院普外科,湖北省武汉市430030 [4]华中科技大学同济医学院附属协和医院神经外科,湖北省武汉市430030

出　　处：《中国临床康复》2005年第17期57-59,共3页Chinese Journal of Clinical Rehabilitation

基　　金：武汉市辰光计划和青年科学家基金资助(2001105047)~~

摘　　要：目的:观察自体颈动脉体细胞团块纹状体内移植对慢性帕金森病猴模型行为学评分的影响,为帕金森病的移植治疗提供实验数据。方法:实验于2003-04/2004-04在同济医学院实验动物中心进行,取4只正常恒河猴,采用肌肉注射1-甲基-4苯基-1,2,3,6四氢吡啶(0.1mg/kg,1次/d)制备慢性帕金森病模型。应用帕金森病猴模型修正评分量表进行评价,行为学症状持续稳定1个月后,应用MRI扫描系统进行移植前定位,取一侧的自体颈动脉体,使用Leksell(GM592)脑立体导向系统行纹状体区立体定向移植术,术后按帕金森病猴模型修正评分量表标准进行行为学评分;并采用阿朴吗啡注射诱发旋转的方法观察治疗效果。结果:所有动物均完成了实验,无死亡脱失。移植后2周,动物开始出现帕金森病猴模型修正评分量表评分进展性下降,至移植后4周,行为学评分与移植前比较出现显著性差异(P<0.05);阿朴吗啡注射后动物出现朝向移植侧的旋转行为。结论:自体颈动脉体细胞团块纹状体内移植能够显著促进帕金森病猴的行为康复。AIM:To observe the effect of intrastriatal autografts of carotid body cell aggregates on behavior rehabilitation in monkeys with chronic Parkinson disease to provide experimental data for the transplantation for treating Parkinson disease.METHODS:The experiment was performed in the Animal Center of Tongji Medical College from April 2003 to April 2004.Four normal rhesus monkeys were intramuscularly injected with 1 Methyl 4 Phenyl 1,2,3,6 Tetrahy dropyridine(0.1 mg/kg per day)to induce chronic Parkinson disease models.Parkinson psychosis rating scale was used to evaluate the behavior of monkeys.With stable behavior for 1 month,the localization was carried out by using magnetic resonance imaging system scanner,unilateral carotid body cell aggregates were transplanted into single striatum with stereo localization technique(Leksell,GM592) and the behavior was evaluated with Parkinson psychosis rating scale after transplantation;Apomorphine was injected to evaluate the recovery of behavior after transplantation.RESULTS:All animal models finished the experiment.Two weeks after transplantation,the monkeys began to show decreased scores on behavior.Until four weeks after transplanted,scores on behavior showed significant difference from those before transplantation(P < 0.05).Apomorphine injections induced revolving behavior toward to the side of the transplantation. CONCLUSION:Intrastriatal autografting of carotid body cell aggregates can accelerate the recovery of behavior in monkeys with Parkinson disease.

关 键 词：帕金森病 猴 颈动脉体/细胞学 移植 疾病模型 动物 

分 类 号：R742.5[医药卫生—神经病学与精神病学]