大鼠海马CA3区内神经元和星形胶质细胞的空间形态(英文)  

Spatial distribution of neurons and astrocytes in CA3 area of rat hippocampus

作　　者：杨志军[1] 徐如祥[1] 饶志仁[2] 魏玲[3] 王晓斌[2] 姜晓丹[1] 

机构地区：[1]南方医科大学珠江医院神经外科,广东省广州市510282 [2]解放军第四军医大学神经科学研究所,陕西省西安市710032 [3]解放军第四军医大学唐都医院呼吸科,陕西省西安市710032

出　　处：《中国临床康复》2005年第17期216-218,共3页Chinese Journal of Clinical Rehabilitation

基　　金：第三十四批中国博士后科学基金资助项目(2003034063);广东省自然科学基金(04300199);国家自然科学基金项目(30400464)~~

摘　　要：背景:星形胶质细胞能够积极参与脑内的神经活动,与神经元之间存在双向的空间信息联系。目的:观察大鼠海马CA3区神经元锥体细胞及其周围星形胶质细胞的分布,重塑两者之间的三维构象。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所大学医院的神经外科、一所军医大学的神经科学研究所。材料:实验2001-10/2003-06在南方医科大学珠江医院神经外科和解放军第四军医大学全军神经科学研究所完成。出生30d的SD雄性大鼠由解放军第四军医大学实验动物中心提供。方法:采用脑片膜片钳全细胞记录、细胞内荧光黄染色、免疫荧光和激光共聚焦显微镜相结合的技术。主要观察指标:主要观察神经元的放电类型及其周围星形胶质细胞的空间分布。结果:根据放电形式的不同主要把海马锥体细胞分为两类:位相型和非位相型放电神经元。激光共聚焦显微镜下单层光学图像和三维立体重建显示许多星形胶质细胞紧密围绕在细胞内荧光黄染色锥体细胞周围并形成紧密接触。两类神经元与星形胶质细胞形成接触的部位存在区别。非位相型放电神经元的树突和胞体周围都有许多星形胶质细胞形成接触的部位,而位相型放电神经元则仅位于树突。结论:不同特性海马神经元周围星形胶质细胞的空间分布可能存在差异。BACKGROUND: Astrocytes play active roles in neuronal activity through reciprocal communication with neurons. OBJECTIVE:To observe the distribution of pyramidal cells and astrocytes in rat hippocampal CA3 area and to reconstruct their three dimensional relationship. DESIGN:A randomized controlled experiment based on rats.SETTING:Neurosurgery department in a university and neuroscience institute in a military medial university of Chinese PLA.MATERIALS:The experiment was conducted in the Neurosurgery Department of Zhujiang Hospital of Southern Medical University and the Neuroscience Institute of Fourth Military Medial University of Chinese PLA from October 2001 to June 2003.Thirty day old male SD rats were provided by the Experimental Animal Center of Fourth Military Medial University of Chinese PLA. METHODS:The techniques used in this study were brain slice patch clamping whole cell recording,lucifer yellow(LY) staining,immunofluorescence and laser scanning confocal microscopy(LSCM). MAIN OUTCOME MEASURES:The discharge features of neurons and the spatial distribution of astrocytes. RESULTS:The hippocampal neurons were classified into 2 types according to their discharge patterns:phasic and non phasic neurons.The observation under both LSCM and three dimensional reconstruction revealed that a large number of astrocytes aggregated around LY dyed pyramidal cells and there were tight contacts between them.The contacts between astrocytes and the two types of neurons differed in that they were found both on dendrites and cell body of non phasic neurons but on dendrites of phasic neurons.CONCLUSION:The spatial distributions of astrocytes around different types of hippocampal pyramids may be different.

关 键 词：海马 神经元 星形细胞 膜片钳术 显微镜检查 共焦 

分 类 号：R74[医药卫生—神经病学与精神病学]