亚低温联合升压治疗对局灶脑缺血再灌注后病灶体积及血脑屏障的影响(英文)  

Effects of hypertension and mild hypothermia on infarct volume and blood-brain barrier in rats after transient focal cerebral ischemia

作　　者：刘传玉[1] 周素荣[2] 李承晏[3] 梅元武[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经科,湖北省武汉市430022 [2]济宁市第一人民医院皮肤科,山东省济宁市272111 [3]武汉大学人民医院神经科,湖北省武汉市430060

出　　处：《中国临床康复》2005年第17期230-232,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:研究表明,亚低温和适当升压对脑缺血都有一定的保护作用,二者联合应用可能产生协同作用,起到更好的脑保护效果。目的:通过升压联合亚低温对大鼠局灶性脑缺血再灌注后脑梗死体积、血脑屏障的影响,探讨脑保护作用机制。设计:以实验动物为研究对象,完全随机对照设计。单位:两所大学医院的神经科和一所市级医院的皮肤科。材料:实验于2001-03/07在华中科技大学同济医学院附属协和医院神经科实验室和武汉大学人民医院神经科实验室完成。选择64只成年Wistar大鼠由武汉大学人民医院实验动物中心提供。干预:制备大鼠局灶性脑缺血模型,随机分为对照组、升压组、亚低温组及升压联合亚低温治疗组(联合组),每组16只动物,缺血3h再灌注;对照组常温不予处理,升压组缺血2h给予升压处理3h,亚低温组缺血2h开始亚低温干预,联合组联合应用亚低温组和升压组治疗方法。缺血24h处死动物。主要观察指标:神经功能缺失评分、脑梗死体积和血脑屏障破坏情况。结果:升压组、亚低温组及联合组的神经功能缺失评分分别为(2.12±0.54)分、(2.14±0.69)分、(1.78±0.61)分,脑梗死体积分别为(17.65±4.78)%,(16.21±3.79)%,(11.31±3.64)%,Even's蓝染色体积分别为(56.63±10.70)mm3,(53.52±8.44)mm3,(38.45±5.25)mm3均明显低于对照组?BACKGROUND:Studies have shown that mild hypertension and hypothermia both offer cerebral protection against focal cerebral ischemia,and their possible synergistic effect may provide even better neuroprotective effects.OBJECTIVE:To investigate the mechanism of cerebral protection by induced hypertension combined with mild hypothermia against focal cerebral ischemia and reperfusion,through observation of the changes in the infarct volume and blood brain barrier(BBB) in rats.DESIGN:A randomized controlled experimental study based on experimental animals. SETTING:The departments of neurology of two university hospitals and department of dermatology in a municipal hospital.MATERIALS:The study was carried out in the Laboratory of Department of Neurology,Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology and Laboratory of Department of Neurology,People's Hospital of Wuhan University from March to July 2001.Sixty four Wistar rats weighing 180 to 230 g were purchased from the Experimental Animal Center of People's Hospital of Wuhan University.INTERVENTIONS:Sixty four rats were randomly divided into control group,hypertension group,mild hypothermia group,and combined therapy group,each group consisting of 16 rats.Reperfusion was initiated after a 3 hour focal cerebral ischemia of the 16 rats,and at 2 hour during the ischemia,the rats in the hypertension and mild hypothermia group were treated with hypertension for 3 hours and mild hypothermia,respectively,and those in the combined therapy group received both treatment.The rats in the control group received no treatments for ischemia and reperfusion.Twenty four hours later,all rats were killed for examination.MAIN OUTCOME MEASURES:The scores of neurological deficits,infarct volume and degree of BBB damage.RESULTS:The scores for neurological deficits,infarct size and volume of Even's blue staining were 2.12± 0.54,(17.65± 4.78)% ,and(56.63 ± 10.70) mm3,respectively,in hypertension group,and 2.14± 0.69,(16.21± 3.79)% ,and(53.

关 键 词：脑缺血 再灌注 低温 血脑屏障 血压 

分 类 号：R743[医药卫生—神经病学与精神病学]