血管紧张素Ⅱ受体在胰腺纤维化大鼠胰腺组织中的表达及其意义  

Expression of angiotensin II receptors after pancreatic fibrosis in rats

作　　者：张汝玲[1] 王兴鹏[1] 吴恺[1] 吴丽颖[1] 董育玮[1] 

机构地区：[1]上海市第一人民医院,上海交通大学附属第一人民医院消化科,上海市200080

出　　处：《中华肝胆外科杂志》2005年第5期328-330,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：上海市科技发展基金资助(No.014119065)

摘　　要：目的动态观察血管紧张素II受体在胰腺纤维化大鼠胰腺组织中的表达并探讨其可能意义。方法采用胰管内注射2%三硝基苯磺酸(TNBS)诱导大鼠胰腺纤维化模型。制模后第3、21、28、35、42、49天分别处死大鼠,每组6只大鼠。对照组仅行剖腹术而未注射TNBS。应用VanGieson(V-G)染色观察胰腺组织纤维化程度。免疫组化和RT-PCR检测胰腺组织AT1蛋白、血管紧张素II受体mRNA和TNF-αmRNA表达。结果模型组大鼠胰腺组织细胞外基质合成较对照组明显增加。胰腺组织TNF-αmRNA表达逐渐增加并于第五周达峰值。AT1、AT2mRNA表达随时间不同呈不同程度的增加,分别于第28天、第35天达到峰值(382%和314%)。与AT1B受体mRNA相比,AT1受体亚型AT1AmRNA表达水平升高更为明显。免疫组化结果提示,模型组大鼠胰腺组织AT1蛋白表达增加并主要分布于纤维化区域。结论血管紧张素II可能通过AT1、AT2介导的途径参与了TNBS诱导的大鼠胰腺组织纤维化形成过程。Objective To investigate the expression of angiotensin Ⅱ receptors in pancreatic tissue and to clarify their effects on development of pancreatic fibrosis in rats.Methods Pancreatic fibrosis was induced in Sprague-Dawley rats by intraductal infusion of 2% trinitrobenenze sulfonic acid(TNBS).The rats with pancreatic fibrosis were sacrificed on day 3,21, 28,42 and 49.Sham-operated rats without infusion of TNBS were employed to serve as the controls.Each group had 6 rats.Van Gieson(V-G) staining was used to assess pancreatic fibrosis.The expression of AT_1 protein,angiotensin Ⅱ receptor mRNA and TNF-α in the pancreatic tissue was detected by either immunohistochemical staining or RT-PCR,respectively.Results As compared with the controls,synthesis of extracellular matrix was increased and expression of TNF-α mRNA was up-regulated in the pancreatic tissue of the rats with TNBS-induced pancreatic fibrosis.The expression of AT_1 and AT_2 mRNA was enhanced in a time-dependent manner,with a maximal expression level of 382% and 314% on day 28 and 35,respectively.In contrast to AT_(1B) mRNA,the expression of AT_(1A) mRNA was significantly up-regulated.The expression of AT_1 protein was also increased in the pancreatic tissue and mainly distributed in the regions of fibrosis.Conclusions Angiotensin Ⅱ is involved in TNBS-induced pancreatic fibrosis probably through AT_1 and AT_2-mediated pathways.

关 键 词：血管紧张素Ⅱ受体 胰腺纤维化 mRNA表达 其意义 表达及 受体MRNA 细胞外基质合成 胰腺组织纤维化 三硝基苯磺酸 TNBS 免疫组化 胰管内注射 纤维化程度 PCR检测 动态观察 诱导大鼠 染色观察 不同程度 AT1B 受体亚型 蛋白表达 

分 类 号：R576[医药卫生—消化系统]