DNFmRNA、GFR-_α1mRNA在不同年龄大鼠SVZ和海马表达的变化及其意义  

Analysis of GDNFmRNA and GFRα_1mRNA gene expression in subventricular zone and hippocampus from Sprague-Dawley rats of different age

作　　者：刘方舟[1] 杨宁[1] 朱岩[2] 曾水林[1] 潘秋辉[3] 刘云龙[1] 

机构地区：[1]东南大学基础医学院人体解剖与组织胚胎学系,江苏南京210009 [2]江苏省人民医院病理科,江苏南京210029 [3]中山大学第二附属医院林百欣实验中心,广东广州510120

出　　处：《东南大学学报（医学版）》2005年第3期171-175,共5页Journal of Southeast University(Medical Science Edition)

基　　金：江苏省自然科学基金资助项目 (BK2 0 0 2 0 56)

摘　　要：目的:观察不同年龄SD大鼠脑室管膜下区(SVZ)和海马GDNFmRNA及其受体GFR- α1mRNA表达水平的变化,探讨其在神经发生及脑老化中的作用。方法:用RT PCR方法检测胚胎18d、新生以及1、3、8、12月龄SD大鼠SVZ和海马GDNFmRNA及其受体GFR -α1mRNA的变化。结果:随着年龄增长,SD大鼠SVZ和海马GDNFmRNA及其受体GFR- α1mRNA表达水平逐渐降低。且各年龄组海马表达参数的幅度小于SVZ。结论:GDNF及其受体GFR- α1在神经发育及脑老化进程中起到一定的作用。Objective To investigate the effect of GDNF and GFRα_1 in the subventricular zone and hippocampus on neural precursor proliferation,via analysing the gene expression of GDNFmRNA and GFRα_1mRNA from Sprague-Dawley rats of different ages. Methods The level of GDNFmRNA and GFR-α_1mRNA in the subventricular zone and hippocampus was observed and compared in rats of different age, using semi-quantitative RT-PCR method with β-actin as an inner consultation. Results With the age increased,the gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular zone and hippocampus was obviously decreased, and the gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular outnumbered those of gene expression in hippocampus of different ages. Conclusions The gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular zone and hippocampus obviously decreases with the increase of age. GDNF and GFRα_1 exert important effects on the neural precursor proliferation, the decline of the neuronal compensatory ability and the neural plasticity of the aged brain.

关 键 词：GDNF 不同年龄 SVZ 海马 其意义 RT-PCR方法 mRNA表达 脑室管膜下区 SD大鼠 神经发生 12月龄 年龄增长 各年龄组 老化进程 神经发育 受体 脑老化 水平 

分 类 号：R651.2[医药卫生—外科学] R541.7[医药卫生—临床医学]