高糖对人晶状体上皮细胞缝隙连接细胞间通讯的影响  

Effect of Glucose in High Concentration on Gap Junction Intercellular Communication Activity in Human Lens Epithelial Cells

作　　者：李霞[1] 刘奕志[1] 吴明星[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060

出　　处：《中山大学学报（医学科学版）》2005年第3期257-259,272,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30070802);教育部博士学科点专项科研基金资助项目(9949)

摘　　要：【目的】研究高糖对人晶状体上皮细胞缝隙连接细胞通讯活性(GJIC)的影响。【方法】将人晶状体上皮细胞(SRA01/04)培养于正常葡萄糖浓度(5.5mmol/L),传代时将其接种于16个35mm的培养皿中,实验分为4组(每组4个培养皿):正常糖浓度组(培养基葡萄糖含量是5.5mmol/L)、高糖组(培养基葡萄糖含量是30mmol/L)、佛波酯(TPA)组(阳性对照)及二甲基亚砜(DMSO)组(阴性对照)。当细胞生长至90%融合时,用划痕荧光染料示踪技术(SL/DT)检测其缝隙连接细胞间通讯活性。【结果】在高糖培养的人晶状体上皮细胞划痕荧光黄向两侧传递的细胞列数为2.23±0.33,明显少于正常糖浓度培养的人晶状体上皮细胞(3.75±0.57,P<0.01)。TPA显著抑制晶状体上皮细胞的GJIC,与正常糖浓度组比较差异具有显著性(1.65±0.26vs3.75±0.57,P<0.01)。【结论】人晶状体上皮细胞缝隙连接细胞间通讯活性在高糖中受到抑制,可能在糖尿病晶状体渗透水肿和离子代谢紊乱中发挥作用。Objective]To investigate the effects of glucose in high concentration on gap junction intercellular communication (GJIC) activity in human lens epithelial cells. [Methods] Human lens epithelial cells (HLECs, SRA01/04) were grown in 16 (35-mm) dishes. And they were divided into 4 groups (Every group containing 4 dishes): normal (5.5 mmol/L) glucose, high (30mmol/L) glucose, 12-O-tetradecanoylphorbol-13-acetate (TPA,positive control) and Dimathyl Sulfoxide (DMSO,negative control). When the HLECs were grown to 90% confluency, the GJIC activity was evaluated by Scrape-Loading/Dye Transfer. [Results] A reduced number of Lucifer Yellow coupled cell layers was observed on either side of the scrape in human lens epithelial cells grown in high-glucose medium compared with the cells grown in normal medium (2.23±0.33 vs 3.75±0.57, P< 0.01). TPA inhibited GJIC activity significantly compared with the group of normal glucose (1.65±0.26 vs 3.75±0.57, P< 0.01). [Conclusion] Glucose in high concentration inhibits GJIC activity in human lens epithelial cells. It may contribute to osmotic damage and abnormal ionic flux in the diabetic lens.

关 键 词：晶状体上皮细胞 糖性白内障 缝隙连接细胞问通讯 划痕荧光染料示踪技术 

分 类 号：R776.1[医药卫生—眼科]