鸭流感病毒4/2004-H6N2亚型毒株的血凝素基因全序列克隆与分析  

The complete nucleotide cloning and sequences of A/Duck/HN/4/2004(H6N2) virus HA and NA

作　　者：顾节清[1] 陈杰[2] 李晓成[2] 杨增岐[3] 吴发兴[2] 张燕霞[2] 黄保续[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]农业部动物检疫所国家动物流行病学中心,青岛266032 [3]西北农林科技大学动物科技学院禽病研究室

出　　处：《中国动物检疫》2005年第5期31-33,共3页China Animal Health Inspection

摘　　要：2004初从正常鸭群中分离到一株鸭源禽流感病毒,命名为A/Duck/HN/4/2004(H6N2)。经对血凝素基因(HA)序列分析发现HA基因全长为1744bp,共编码566个氨基酸,在裂解位点仅含一个碱性氨基酸-精氨酸(R),符合LPAIV的标准。将所得基因序列与已发表的同一亚型参考序列分析表明,与H6亚型流感HA基因同源性为89.2%～97.1%,经分子遗传演化分析表明本次分离株与香港分离株A/Duck/HongKong/3600/99(H6N2)、A/Duck/HongKong/3600/99(H6N2)最近。An avian influenza virus (AIV) was isolated from a duck flock without any sign of clinical disease in early 2004, and named as A/Duck/HN/4/2004(H6N2). Squencing of the HA fragment showed that the full length of HA gene is 1744bp,covering the HA gene of the open reading frame,encoding 566 amino acid ,including 344 amino acid of HA1 and 221 amino acid of HA2.Compared with the same subtype sequence from GenBank ,their nucleotide homology of hemagglutinin was from 89.2% to 97.1% with H6 subtype hemmagglutinin.The phylogenetic relationships showed that the A/Duck/HN/4/2004(H6N2) was closest to the isolates (H6N2) from HongKong(A/Duck/HongKong/3600/99(H6N2)?A/Duck/HongKong/3600/99(H6N2)).

关 键 词：血凝素基因 亚型 全序列 克隆 毒株 禽流感病毒 碱性氨基酸 基因同源性 HA基因 序列分析 裂解位点 参考序列 基因序列 遗传演化 分析表 分离株 精氨酸 鸭源 鸭群 全长 

分 类 号：S852.659.5[农业科学—基础兽医学] S436.429[农业科学—兽医学]