中国特发性无精子症和少精子症患者雄激素受体基因CAG重复多态性研究  

(CAG)n Polymorphism of Androgen Receptor Gene in Idiopathic Azoospermic and Oligospermic Chinese Men

作　　者：李座祥[1] 唐文豪[2] 汪朝晖[3] 王琳[3] 王媛媛[1] 马潞林[2] 马旭[1] 

机构地区：[1]中国协和医科大学,北京100730 [2]北京大学第三医院泌尿外科,北京100083 [3]国家人口计生委科学技术研究所遗传室,北京100081

出　　处：《中华男科学杂志》2005年第5期335-338,342,共5页National Journal of Andrology

基　　金：国家重点基础研究发展规划(973计划);中国生殖健康生物信息库的建立和应用研究项目(G1999.055905)资助

摘　　要：目的分析中国特发性无精子症和少精子症患者雄激素受体(AR)基因(CAG)n微卫星多态性并探讨该多态性与精子生成障碍发生的关系。方法应用PCR和变性聚丙烯酰胺凝胶电泳分析技术对52例少精子症患者和31例无精子症患者的外周血标本进行CAG重复数测定,分析该微卫星多态性和精子生成障碍发生的关系。结果少精子症患者组和无精子症患者组CAG重复数均数分别为22.19和22.13,CAG重复数≥28的百分率分别为1.9%和3.2%,比例逐渐升高。结论AR基因(CAG)n微卫星的CAG重复数在中国男性不育患者中呈现多态性,与精子生成障碍发生的关系有待进一步研究。Objective: To research (CAG)n polymorphism of androgen receptor gene in idiopathic azoospermic and oligospermic Chinese men, and to explore its correlation with the development of dyszoospermia. Methods: Fifty-two men with oligospermia and 31 with azoospermia were enrolled, the number of CAG sequence repeats determined by PCR and denaturing polyacrylamide gel electrophoresis (PAGE), and (CAG)n polymorphism analyzed. Results: The CAG repeats in the men with oligospermia and those with azoospermia were ~22.19 and ~22.13 respectively. Conclusion: The polymorphism of the repeat length of the (CAG)n satellite in androgen receptor gene may affect spermatogenesis and have a less important role in male infertility.

关 键 词：男性不育 雄激素受体基因 CAC重复序列 基因多态性 

分 类 号：R698.2[医药卫生—泌尿科学] R394.3[医药卫生—外科学]