肠球菌部分致病基因和表型的检测  

Detection of partial virulence determinants and phenotypes in Enterococci

作　　者：马立艳[1] 许淑珍[1] 马纪平[1] 

机构地区：[1]首都医科大学附属北京友谊医院检验科,100050

出　　处：《中华检验医学杂志》2005年第5期529-532,共4页Chinese Journal of Laboratory Medicine

基　　金：北京市自然科学基金项目资助(7012013)

摘　　要：目的　调查临床分离的肠球菌6种致病基因和2种表型的分布。方法　应用聚合酶链反应和斑点杂交技术检测临床分离的145株肠球菌的6种致病基因,用7%兔血平板和3%明胶平板检测β溶血和明胶溶解表型。结果　粪肠球菌和屎肠球菌6种致病基因的检出率分别为gelE72 9%、30 .6%; efaA 79. 2%、36 .7%; cylA 54. 2%、34 .7%; esp 34 .4%、36. 7%; agg 18 .8%、0;ace28. 1%、0。粪肠球菌和屎肠球菌β溶血分别为45. 8%、20 .4%;明胶溶解分别为35 .4%、16 .3%。结论　粪肠球菌6种致病基因和2种表型的检出率高于屎肠球菌;尿标本比痰标本肠球菌致病基因的检出率高;gelE、efaA和cylA在粪肠球菌中的检出率高于其他致病基因。Objective To investigate the incidence of six virulence determinants and two phenotypes in clinical strains of Enterococci.Methods PCR and dot blot were used to detect the six virulence determinants in 145 clinical isolates. The two phenotypes , including β-hemolysis and gelatin hydrolyze ,were performed on agar plate containing 7% rabbit blood and Todd-Hewitt agar plate supplemented with 3% gelatin, respectively.Results In E. faecalis and E. faecium ,the rates of detection of the six virulence determinants were gelE72.9% vs 30.6%,efaA79.2% vs 36.7%,cylA54.2% vs 34.7%,esp34.4% vs 36.7%,agg 18.8% vs 0, ace28.1% vs 0, respectively; the β hemolysis was 45.8% vs 20.4%,the gelatin hydrolyze was 35.4% vs 16.3%.Conclusions E. faecalis possess more virulence determinants and phenotypes than E. faecium do. The virulence determinants are more common among the strains isolated from urine sample than those from among sputum . Most of the E. faecalis carry virulence determinants of gelE,efaA and cylA .

关 键 词：致病基因 表型 粪肠球菌 聚合酶链反应 屎肠球菌 临床分离 检出率 技术检测 斑点杂交 血平板 明胶 esp 痰标本 尿标本 溶解 

分 类 号：R446.5[医药卫生—诊断学]