florisil柱净化玉米、水稻及糙米中黄曲霉毒素的最优化条件研究  

Optimization Condition of Aflatoxin Purification from Corn, Paddy Rice and Brown Rice Using Florisil Column

作　　者：高军侠[1] 万昊雷[2] 刘作新[1] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]沈阳农业大学生物科技学院,沈阳110161

出　　处：《郑州大学学报（理学版）》2005年第2期101-103,108,共4页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家粮食丰产科技工程;编号2004BA520A01-5;中国科学院沈阳应用生态研究所海外学者基金;编号SCXLX0204;辽宁省"十五"科技攻关招标项目;编号2001212001.

摘　　要：研究了利用florisil柱净化玉米、水稻及糙米中黄曲霉毒素的最优化条件.两种粒度的florisil填充柱净化玉米均较理想,但粒度150～250μm的florisil对水稻及糙米的净化影响较大,建议选用较小粒度的florisil材料.大粒度的florisil能减小溶剂的流速,用不同的溶剂淋洗杂质,最佳流速有所不同,氯仿-正己烷(体积比1∶1)的最佳流速是1.55mL·min-1,而氯仿-甲醇(体积比9∶1)的最佳流速是0.68mL·min-1.The optimization conditions by florisil column for purifying aflatoxins from corn, paddy rice and brown rice are studied.Two meshes are ideal to purify corn using florisil column. However, the less mesh has a significant effect when purifying aflatoxins from paddy rice and brown rice, and it is advised that the larger mesh be used to purify them. The less mesh florisil can minish solvent velocity. The best velocities of flow vary with different solvents in scouring impurity off. The optimization washing velocity of chloroform-hexone is about 1.55 mL·min -1, while that of chloroform-methanol is 0.68 mL·min -1.

关 键 词：最优化条件 黄曲霉毒素 净化 玉米 糙米 水稻 最佳流速 体积比 填充柱 大粒度 小粒度 min 正己烷 溶剂 氯仿 理想 

分 类 号：O224[理学—运筹学与控制论] TS207.4[理学—数学]