固定化酵母细胞生产1，6－二磷酸果糖的动力学研究  

Studies on Kinetics of Immobilized Yeast Cells for Preparation of Fructose-1,6-Diphosphate

作　　者：王丁刚[1] 吴梧桐[1] 张宏兴 王旻[1] 齐燕明 杜振宁[1] 

机构地区：[1]中国药科大学生物化学教研室

出　　处：《中国药科大学学报》1994年第5期297-299,共3页Journal of China Pharmaceutical University

摘　　要：筛选获得一株１，６－二磷酸果糖（ＦＤＰ）的高产酵母菌株，改变通透性后的酵母细胞，在发酵及固定化生产ＦＤＰ过程中发现Ｆｅ２＋和甘油能大量提高ＦＤＰ的积累。将戊二醛交联后的酵母细胞，用卡拉胶固定化后，装入酶反应柱，连续生产ＦＰＤ，柱反应可稳定在１０天以上，转化率维持在２０％以上。A strain of yeast has been selected for preparation of fructose-1,6-diphcophate(FDP).Continuous preparation of FDP was attempted by means of glutaraldehyde cross linked yeast cells immobilized by carrageenin in column reactor, the influence of velocity of substrate solution, pH,T,Fe2+ and glycerol on FDP accumulation was studied. The steady level of FDP production was maintained at least 10 days in column reactor.

关 键 词：二磷酸果糖 固定化 酵母细胞 动力学 

分 类 号：TQ464.1[化学工程—制药化工]