β-环糊精用于阿莫西林的二次微分简易示波伏安测定  

Application of β-Cyclodextrin to Determination of Amoxicillin by Second Order Differential Simple Oscillographic Voltammetry

作　　者：郑建斌[1] 陶福芳[1] 

机构地区：[1]西北大学分析科学研究所,陕西省电分析化学重点实验室,西安710069

出　　处：《应用化学》2005年第6期595-599,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20275030);陕西省自然科学基金资助项目(2004B20)

摘　　要：利用β环糊精(βCD)与阿莫西林在0.2mol/LNaOH底液中形成包结物而使βCD在二次微分简易示波伏安图上峰高降低的示波特性,建立了胶囊中阿莫西林的二次微分简易示波伏安测定新方法并测定了βCD与阿莫西林的包络比和包结常数。测定阿莫西林的线性范围为2.5×10-6～3.0×10-5mol/L,回归方程为h(ν)=4.07-1.47×104c(mol/L),r=-0.9979,检出限为1.0×10-6mol/L。对1.5×10-5mol/L阿莫西林进行5次测定的相对标准偏差(RSD)为2.3%,回收率为100.1%。另外,测得βCD与阿莫西林形成包结物的包结比为1∶1、包结常数为6.0×105。β-Cyclodextrin(β-CD) exhibits a pair of reversible peaks in the second order differential simple oscillgraphic voltammogram in a 0.2 mol/L NaOH solution, and the peak height decreases with the increase in the concentration of the inclusion complex of amoxicillin-β-CD. Based on the oscillographic characteristic of amoxicillin-β-CD, a second order differential simple oscillgraphic voltammetry method for determination of amoxicillin in capsule is presented. A linear response was observed in the range of 2.5×10 -6～3.0× 10 -5 mol/L with a correlation coefficient of -0.997 9 and a detection limit of 1.0×10 -6 mol/L for amoxi- cillin. The relative standard deviation(RSD) for the determination of 1.5×10 -5 mol/L amoxicillin was 2.3%(n=5), and the average recovery rate was 100.1%(n=5). In addition, the inclusion ratio and inclusion constant(K) of amoxicillin-β-CD determined by the Job method were 1∶1 and 6.0×10 5, respectively.

关 键 词：药物分析 示波分析 示波测定 Β-环糊精 阿莫西林 

分 类 号：O657.1[理学—分析化学]